Accepted by Department Council Made by Assoc. Prof. Y. Koeva, md, PhD

Lectures and laboratory seminars in Cytology, Histology and Embryology І course, medical students, winter semester 2012/2013

1.Lipid drops

2.Glycogen granules in hepatic cells

3.Pigment inclusions

1. 
   Cell membrane
   
2. 
   Microvilli
   
3. 
   Desmosomes
   
4. 
   Interdigitations
   
5. 
   Protein granules
   
6. 
   Lipid drops
   
7. 
   Glycogen granules
   

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1. 
   Nissl substance (granules)
   
2. 
   Mitochondria
   
3. 
   SDH - activity
   
4. 
   Golgi complex
   

1.Rough endoplasmatic reticulum

2.Smooth endoplasmatic reticulum

3.Ribosomes

4. Мitochondria

5.Golgi complex

6.Centrosome

1. 
   Cytoskeleton
   
2. 
   Specialized cell organelles
   

1.Еndoplasmatic reticulum

2.Мitochondria

3.Golgi complex

4.Lysosomes

1. Nucleus in interphase

1. 
   H-E staining
   
2. 
   Foilgen staining
   
3. 
   DNA synthesis (histoautoradiography)
   

1. Interphase nucleus

1. 
   Phagocytosis
   
2. 
   Acid phosphatase activity
   
3. 
   Secretion (secretory granulea)
   
4. 
   Mooving of cillia – demonstration
   

1. 
   Cilia
   

1.2. Transverse section

2. 
   Secretory granules
   

2.Acid phosphatase activity

3.Secretion

4.Mooving of cilia

1. 
   Simple squamous epithelium
   
   1. 
      Н-Е staining
      
   2. 
      Impregnation
      
2. 
   Cuboidal epithelium
   
3. 
   Columnar epithelium
   
4. 
   Unilayered (pseudostratified) ciliated columnar epithelium
   

1. 
   Histogenesis
   
2. 
   General characteristics
   
3. 
   Classification
   

5 microscopical preparations

5 - electron microphotographs 20 points

2.Тheoretical part – test

40 points

60 points total

Discharge: 70% /14 p. + 28 p./

1. 
   Histogenesis
   
2. 
   General characteristics
   
3. 
   Classification
   
4. 
   Cells.
   
5. 
   Intercellular substance
   
6. 
   Fibrous connective tissue
   

1. 
   Stratified squamous epithelium
   
2. 
   Stratified squamous keratinizing epithelium
   
3. 
   Secretory epithelia
   

3.2.Serous, mucous and compound alveolar (acinar) glands

1. Cartilage

2. The bone tissue

1.Loose connective tissue

2.Collagenous fibrous tissue

3.Ellastic tissue

4.Pigment connective tissue

5.Adipose tissue – white and brown

a)Sudan III staining

b)H-E staining

1. 
   Fibroblast
   
2. 
   Mast cell
   
3. 
   Plasma cell
   
4. 
   Collagenfibers
   

1. 
   Histogenesis
   
2. 
   General characteristics
   
3. 
   Classification
   
4. 
   Cells
   

4.2.Leucocytes

4.3.Platelets

1. Cartilage

2. The bone tissue

Microscopical preparations:

1. 
   Hyaline cartilage
   
2. 
   Elastic cartilage
   
3. 
   Compact bone – decalcinated
   
4. 
   Compact bone – Shliff
   

1. 
   Оsteocyte
   

1. 
   Histogenesis
   
2. 
   General characteristics
   
3. 
   Classification
   

5. Myofibers, myofibrils

1. 
   Blood smear - preparation
   
2. 
   Blood smear - examination
   

1. 
   Neotrophil granulocyte
   
2. 
   Еоsinophil granulocyte
   
3. 
   Lymphocyte
   

1.Histogenesis

2.General characteristics

3.Classification

4.Cells – neurons, neuroglia

5. Nerve fibers

6. Myoneural synapse

1. 
   Smooth (visceral) muscle tissue
   
2. 
   Striated Skeletal muscle tissue
   
3. 
   Striated Cardiac muscle tissue
   
4. 
   Impulse conductive cardiac muscle tissue
   

1. 
   Myofibril – skeletal
   
2. 
   Cardiomyocyte – myofibril
   

2.General characteristics

3.Classification

4.Cells

4.1. Oocytes

4.2. Spermatozoa

1. 
   Мultipolar neurons
   
2. 
   Pear-like neurons
   
3. 
   Pyramidal neurons
   

1. 
   Myelinated nerve fiber
   

1. 
   Oocyte
   
2. 
   Spermatozoa
   
3. 
   Early gastrulation
   
4. 
   Late grastrulation
   

1. 
   Oocyte
   
2. 
   Spermatozoon
   

1. 
   Chorion, amnion, yolk sack, alantoic diverticulum
   
2. 
   Umbilical cord
   
3. 
   Fetal sack
   
4. 
   Placenta
   

1. 
   Umbilical cord of 2-3 month old human embryo
   
2. 
   Umbilical cord of newborn baby
   
3. 
   Placenta