Accepted by Department Council Made by Assoc. Prof. Y. Koeva, md, PhD



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Accepted by Department Council










Made by Assoc. Prof. Y. Koeva, MD, PhD




SYLLABUS

Lectures and laboratory seminars in Cytology, Histology and Embryology І course, medical students, winter semester 2013






Weeks

Laboratory seminars - 3 h.

Prof. P. Atanassova

Lectures - 2 h

Prof. P. Atanassova

І week

-----------------------

ІІ week



Cytoplasma. Cell inclusions
Microscopical preparations:

1.Lipid drops

2.Glycogen granules in hepatic cells

3.Pigment inclusions



Еlectron microphotographs:

  1. Cell membrane

  2. Microvilli

  3. Desmosomes

  4. Interdigitations

  5. Protein granules

  6. Lipid drops

  7. Glycogen granules

----------------------------------------



Cytoplasm. Cell organelles. Microscopical preparations:

  1. Nissl substance (granules)

  2. Mitochondria

  3. SDH - activity

  4. Golgi complex

Еlectron microphotographs:

1.Rough endoplasmatic reticulum

2.Smooth endoplasmatic reticulum

3.Ribosomes

4. Мitochondria

5.Golgi complex

6.Centrosome


The cell

Cell – morphology.

Cell membrane (plasmalema)

Cytoplasm, cytosol
Cytoplasm.

  1. Cytoskeleton

  2. Specialized cell organelles

Cell inclusions

-----------------------------------

Cell orgenelles

General organelles- structural, ultrastructural and functional characteristics.

1.Еndoplasmatic reticulum

2.Мitochondria

3.Golgi complex

4.Lysosomes





ІІІ week


Nucleus in interphase. Mitosis.

Microscopical preparations:

1. Nucleus in interphase



    1. H-E staining

    2. Foilgen staining

    3. DNA synthesis (histoautoradiography)

2.Mitosis in cells of pea radix

Еlectron microphotographs:

1. Interphase nucleus



Nucleus in interphase and mitosis.
1. Interphase nucleus - structural, ultrastructural and functional characteristics.
2.Mitosis – phases




ІV week


Cytophysiology
Microscopical preparations:

  1. Phagocytosis

  2. Acid phosphatase activity

  3. Secretion (secretory granulea)

  4. Mooving of cillia – demonstration


Еlectron microphotographs:

  1. Cilia

1.1 Longitudinal section

1.2. Transverse section



  1. Secretory granules




Cytophysiology
1.Phagocytosis

2.Acid phosphatase activity

3.Secretion

4.Mooving of cilia






V week


Epithelia. Unilayered epithelia
Microscopical preparations:

  1. Simple squamous epithelium

    1. Н-Е staining

    2. Impregnation

  2. Cuboidal epithelium

  3. Columnar epithelium

  4. Unilayered (pseudostratified) ciliated columnar epithelium

5. Henle’s epithelium

Tissues – classification,properties
Epithelia.

Unilayered epithelia

Multistratified epithelia


  1. Histogenesis

  2. General characteristics

  3. Classification







VІ week


Colloquium

(preliminary examination)

1. Practical part

5 microscopical preparations

5 - electron microphotographs 20 points

2heoretical part – test

40 points

60 points total

Discharge: 70% /14 p. + 28 p./


Connective tissue.

  1. Histogenesis

  2. General characteristics

  3. Classification

  4. Cells.

  5. Intercellular substance

  6. Fibrous connective tissue




VІІ week


Epithelia.

Multistratified epithelia. Secretory epithelia.

Microscopical preparations:

  1. Stratified squamous epithelium

  2. Stratified squamous keratinizing epithelium

  3. Secretory epithelia

3.1.Simple tubular glands

3.2.Serous, mucous and compound alveolar (acinar) glands



Connective tissue with solid intercellular substance.

1. Cartilage

2. The bone tissue


VІІІ week



Fibrous connective tissue

Microscopical preparations:

1.Loose connective tissue

2.Collagenous fibrous tissue

3.Ellastic tissue

4.Pigment connective tissue

5.Adipose tissue – white and brown

a)Sudan III staining

b)H-E staining



Еlectron microphotographs:

  1. Fibroblast

  2. Mast cell

  3. Plasma cell

  4. Collagenfibers

5. Multilocular adipocyte

Blood tissue.

  1. Histogenesis

  2. General characteristics

  3. Classification

  4. Cells

4.1.Еrythrocytes

4.2.Leucocytes

4.3.Platelets





ІХ week


Connective tissue with solid intercellular substance.

1. Cartilage

2. The bone tissue

Microscopical preparations:


  1. Hyaline cartilage

  2. Elastic cartilage

  3. Compact bone – decalcinated

  4. Compact bone – Shliff

Еlectron microphotographs:

  1. Оsteocyte

2. Оsteoclast

Мuscle tissue.

  1. Histogenesis

  2. General characteristics

  3. Classification

4. Cells

5. Myofibers, myofibrils




X week



Blood tissue.

Microscopical preparations:

  1. Blood smear - preparation

  2. Blood smear - examination

Еlectron microphotographs:

  1. Neotrophil granulocyte

  2. Еоsinophil granulocyte

  3. Lymphocyte

Test – 30 p. and preparations –10 p./8+2/ on Epithelial and Connective tissues.

Total score – 40 p.


Nerve tissue.

1.Histogenesis

2.General characteristics

3.Classification

4.Cells – neurons, neuroglia

5. Nerve fibers

6. Myoneural synapse


ХI week


Мuscle tissue.

Microscopical preparations:

  1. Smooth (visceral) muscle tissue

  2. Striated Skeletal muscle tissue

  3. Striated Cardiac muscle tissue

  4. Impulse conductive cardiac muscle tissue

Еlectron microphotographs:

  1. Myofibril – skeletal

  2. Cardiomyocyte – myofibril





Reproductive tissue 1.Histogenesis

2.General characteristics

3.Classification

4.Cells

4.1. Oocytes

4.2. Spermatozoa



ХІI week


Nerve tissue.

Microscopical preparations:

  1. Мultipolar neurons

  2. Pear-like neurons

  3. Pyramidal neurons

4. Myelinated nerve fibers

Еlectron microphotographs:

  1. Myelinated nerve fiber

2. Myoneural synapse



Early human embryonal development.
Fertilization

Clivage

Blastocyst

Implantation

Gastrulation

Primitive organs


ХІІI week


Reproductive tissue.

General embryology. Gastrulation.

Microscopical preparations:

  1. Oocyte

  2. Spermatozoa

  3. Early gastrulation

  4. Late grastrulation

Еlectron microphotographs:

  1. Oocyte

  2. Spermatozoon

Теst 40 p. and preparations -10 p./6+4/ on blood, muscle and nerve tissues

Total score - 50 p.

Extra-embryonic layers

  1. Chorion, amnion, yolk sack, alantoic diverticulum

  2. Umbilical cord

  3. Fetal sack

  4. Placenta




ХІV week


Extra-embryonic layers

Microscopical preparations:

  1. Umbilical cord of 2-3 month old human embryo

  2. Umbilical cord of newborn baby

  3. Placenta

4. Fetal sack

Twins

Anomalies in the human embryonal development


1.Colloquium in Cytology – 60 points

2.Test on epithelial and connective tissues – 40 points

3. Test on blood, muscle and nerve tissues – 50 points

Total score points for the semester – 150

Exellent 6,00 – more than 90% more than 135p.

Very good 5,00 - 80% 120-134 p.

Good 4,00 - 70% 105-119 p.

Satisfactiry 3,00 - 60% 90-104 p.

Poor - less than 90 points



Textbooks

  1. Basic Histology. I.C. Janqueira

  2. Textbook of Human Histology, I. Singh

  3. Human embryology , Inderbir Singh, Sixth edition

  4. Clinical and Functional Histology for Medical Students, Richard S. Snell

  5. Practicum of Cytology, Histology and Embryology with CD , P. Atanassova, Y. Koeva, E. Petrova, N. Penkova, V. Trichkova

  6. Handbook in cytology, histology and embryology. Assoc. Prof. Yvetta Koeva.

Made by Assoc. Prof. Y. Koeva, PhD

I медици

Колоквиум по цитология:

Студентите, получили 80% от точките на практическата част (16 точки) и на теста (32 точки) от колоквиума по цитология се освобождават от раздел цитология на изпита по цитология, обща ембриология и хистология.

Студентите, получили 70% от точките на практическата част (14 точки) и на теста (28 точки) от колоквиума по цитология се освобождават от практическата част (микроскопски препарати) по цитология на изпита по цитология, обща ембриология и хистология.
Семестриален успех:

Студентите, получили 90% от точките за целия семестър (над 135 точки) се освобождават от входящия тест (микроскопски препарати и теоретичен тест) на изпита по цитология, обща ембриология и хистология. Същите се явяват на писмен и устен изпит.







Каталог: files -> 2013
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2013 -> Тридневна слънчево-геофизическа прогноза за периода 5 – 7 ноември 2013 Състояние на слънчевата активност
2013 -> Тридневна слънчево-геофизическа прогноза за периода 16 – 18 януари 2013 Състояние на слънчевата активност
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