Секция Микробна генетика лаборатория по генетика на млечно кисели бактерии ръководител: доц д-р Светла Трифонова Данова

Гл. ас. д-р Йордан Манасиев

Росица Тропчева- докторант
Област на изследвания: МЛЕЧНО КИСЕЛИ БАКТЕРИИ и ПРОБИОТИЦИ
Анотация:

Mолекулярно-генетични изследвания на млечно-кисели бактерии (МКБ): видова идентификация с помощта на PCR, ДНК хибридизационни и други високо дисктиминативни молекулярни методи, секвенционен и филогенетичен анализ; типизиране и генeтично паспортизиране на новоизолирани и колекционни щамове; плазмиди, гени и молекулни механизми на полезни и здравословни ефекти.

Биологична активност, пробиотични свойства и лабораторна оценка на технологично значими свойства на млечно-кисели бактерии.
Постижения:

За първи път е характеризирана нестартерната микрофлора на традиционни български млечни продукти със съвременни молекулярни подходи и е получена информация за биоразнообразието на лактобацилните видове в бяло саламурено сирене и катък и е оценен техния пробиотичен потенциал и възможности за приложението им във функционални храни.

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  R. Georgieva, R. Tropcheva, P. Koleva, D. Nikolova, J. Manassiev, S. Antonova-Nikolova, G. Dobreva-Yosifova, Y. Evstatieva, V. Paskov, S. Danova „Traditional Bulgarian chesses and beneficial lactic acid microflora”, Proceedings of the 1^st international symposium on “Traditional foods from Adriatic to Caucasus”, 15-17 April 2010, Tekirdag, Turkey, pp. 320-322.
  
• 
  R. Tropcheva, D. Nikolova, Y. Evstatieva, R. Georgieva, S. Danova “Antifungal Activity of Lactobacillus strains from traditional Bulgarian dairy products”, Proceedings of the 1^st international symposium on “Traditional foods from Adriatic to Caucasus”, 15-17 April 2010, Tekirdag, Turkey, pp. 46-48.
  
• 
  R. Georgieva, I. Iliev, T. Haertlé, J-M Chobert, I. Ivanova, S. Danova. Technological properties of candidate probiotic Lactobacillus plantarum strains, International Dairy Journal, 2009, 696-702
  
• 
  S. Danova, R. Georgieva, P. Koleva, R. Tropcheva, J. Manasiev, D. Nikolova. Biodiversity and activity of Lactic acid bacteria from traditional Bulgarian milk products, Proceeding of International Scientific conference: Food Science, Engeenering And Technologies, 23-24 October 2009, Plovdiv, vol LVI, Issue 1, 275-280
  
• 
  S. P. Stoylov, A. Gyurova, V. Bunin, A. Angersbach, R. Georgieva, S. Danova. Electric dipole moments of E. coli HB 101”, Bioelectrochemistry, 2009, 75, 50–54
  
• 
  R. Georgieva, P. Koleva, D. Nikolova, D. Yankov, S. Danova^{, “}Growth parameters of probiotic strain Lactobacillus plantarum, isolated from traditional white cheese”. Biotechnology & Biotechnological Equipment, 2009, 23SE, 861-865
  
• 
  D. Nikolova, Y. Evstatieva, R. Georgieva, S. Danova, V. Savov, S. Ilieva, P. Dalev, Molecular taxonomic characterisation of probiotic strain Lactobacillus sp. 50P1, 2009, 23SE, 779-782
  
• 
  M. Petrova, R. Georgieva, S. Dimitonova, N. Ivanovska, N. Hadjieva and S. Danova, “Inhibitory activity of vaginal lactobacilli against human pathogens”, Biotechnology & Biotechnological Equipment, 2009, 23SE, 627-631
  
• 
  P. Koleva, R. Georgieva, D. Nikolova, S. Danova, “Lactic acid microflora of Bulgarian milk products ^{Biotechnology & Biotechnological Equipment 2009, 23SE, 856-860}
  
• 
  M. Petrova, R. Georgieva, L. Dojchinovska, N. Kirilov, I. Iliev, S. Antonova, N. Hadjieva, I. Ivanova, S. Danova, Lactic Acid Bacteria against pathogenic microbes. Tracia Journal of Sciences, 2009, 7 (2), 33-39
  
• 
  G. Dobreva-Yosifova, L. Yocheva, A. Mehmed, S. Danova and S. Antonova-Nikolova. In vitro characterization of probiotic properties of lactic acid bacteria from Bulgarian rye sourdoughs Biotechnology & Biotechnological Equipment, 2009, 23SE, 801-805
  
• 
  N.Kirilov, T. Petkova, J. Atanasova, Sv. Danova, I. Iliev, Y. Popov, T. Haertle and I. V. Ivanova. Proteolytic activity in lactic acid bacteria from iraq, armenia and bulgaria Biotechnology & Biotechnological Equipment, 2009, 23SE, 643-646
  
• 
  S.P. Dimitonova, B.V. Bakalov, R.N. Aleksandrova-Georgieva, S.T. Danova, Phenotypic and molecular identification of vaginal Lactobacillus isolates from Bulgarian women. Journal of Microbiology, Immunology and Infection, 2008, 41, 469-477
  
• 
  S. P. Stoylov, A. Gurova, R. Georgieva, S. Danova Do bacteria have an electric permanent dipole moment? Colloids and Surfaces B: Biointerfaces, 2008, 64, 255–259
  
• 
  R. N. Georgieva, I. N. Iliev, V. A. Chipeva^,, S. P. Dimitonova, J. Samelis, S.T. Danova, Identification and in vitro characterisation of L. plantarum strains from artisanal Bulgarian white brined cheeses, Journal of Basic Microbiology, 2008, 48, 234-244
  
• 
  Silviya P. Dimitonova, Boris V. Bakalov, Julia P. Serkedjieva and Svetla T. Danova. Inhibitory activity and protective properties of vaginal lactobacilli from healthy Bulgarian women. Anaerobe, 2007, 13 178–184
  
• 
  Todorov S.D., Botes M., Danova S.T. and Dicks L.M.T. Probiotic properties of Lactococcus lactis subsp. lactis HV219, isolated from human vaginal secretions. Journal of Applied Microbiology, 2007, 103, 629-639
  
• 
  Irena Tzvetkova, Michèle Dalgalarrondo, Svetla Danova, Ilia Iliev, Thomas Haertlé, Iskra Ivanova and Jean-Marc Chobert. Hydrolysis of major dairy proteins by lactic acid bacteria from Bulgarian yogurts. Journal of Food Biochemistry 2007, 31 (5), 680-702
  
• 
  Galina D. Stoyancheva, Svetla T. Danova and Ivo Y. Boudakov. Molecular identification of vaginal lactobacilli isolated from Bulgarian women, Antonie van Leeuwenhoek International journal of general and molecular microbiology 2006, OCT 2006, 90 (3): 201-210
  
• 
  Lidiya Angelova, Michèle Dalgalarrondo, Ignat Minkov, Svetla Danova, Nicolai Kirilov, Julia Serkedjieva, Jean-Marc Chobert, Thomas Haertlé, Iskra Ivanova. Purification and characterisation of a protease inhibitor from Streptomyces chromofuscus 34-1 with an antiviral activity. Biochimica et Biophysica Acta GS, 2006, AUG 1760 (8): 1210-1216
  
• 
  Todorov Svetoslav D., Stoyancheva Galina D., Martina Meincken, Danova Svetla T. and Dicks Leon M.T. Characterization of bacteriocin HV219, produced by Lactococcus lactis subsp. lactis HV219 isolated from human vaginal secretions, Journal of Basic Microbiology 2006, Vol. 46, No. 3, 226-238
  
• 
  S.T. Danova, G. Stoyancheva, Y. Manasiev and V. Miteva, Development and application of random DIG labelled probes based on total genomic Lactobacillus DNA”, World Journal of Microbiology & Biotechnology, 2005, 21: 835-842
  
• 
  Svetla Danova, Kaloyan Petrov, Plamen Pavlov, Penka Petrova Isolation and characterization of Lactobacillus strains involved in koumiss fermentation”, International Journal of Dairy Technology, 2005, vol. 58, No 2 May, 100-105
  

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  2008-2011: “Molecular characterization, immunomodulation activity and mechanisms of healthy benefits of original lactobacilli from different ecological niches” DOO2-187- research project with National Scientific found, Bulgaria-.
  
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  2007-2011 “Study of antimicrobial and hypoallergenic products of lactic acid bacteria” research NATO, coordinated by INRA-Nantes (France) and Biological faculty-Sofia University.
  
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  2008-2011 “Confocal laser-scanning microscopy-for evaluation of Bulgarian bioresurses”, NSF, BULGARIA-2009-2011-Coordinated by Department of cell morphology, Institute of Microbiology, BAS.
  

1. 
   R. Georgieva, R. Tropcheva, P. Koleva, D. Nikolova, J. Manassiev, S. Antonova-Nikolova, G. Dobreva-Yosifova, Y. Evstatieva, V. Paskov, S. Danova „Traditional Bulgarian chesses and beneficial lactic acid microflora”, Proceedings of the 1^st international symposium on “Traditional foods from Adriatic to Caucasus”, 15-17 April 2010, Tekirdag, Turkey, pp. 320-322.
   
2. 
   R. Tropcheva, D. Nikolova, Y. Evstatieva, R. Georgieva, S. Danova “Antifungal Activity of Lactobacillus strains from traditional Bulgarian dairy products”, Proceedings of the 1^st international symposium on “Traditional foods from Adriatic to Caucasus”, 15-17 April 2010, Tekirdag, Turkey, pp. 46-48.
   
3. 
   R. Georgieva, I. Iliev, T. Haertlé, J-M Chobert, I. Ivanova, S. Danova. Technological properties of candidate probiotic Lactobacillus plantarum strains, International Dairy Journal, 2009, 696-702
   
4. 
   S. Danova, R. Georgieva, P. Koleva, R. Tropcheva, J. Manasiev, D. Nikolova. Biodiversity and activity of Lactic acid bacteria from traditional Bulgarian milk products, Proceeding of International Scientific conference: Food Science, Engeenering And Technologies, 23-24 October 2009, Plovdiv, vol LVI, Issue 1, 275-280
   
5. 
   S. P. Stoylov, A. Gyurova, V. Bunin, A. Angersbach, R. Georgieva, S. Danova. Electric dipole moments of E. coli HB 101”, Bioelectrochemistry, 2009, 75, 50–54
   
6. 
   R. Georgieva, P. Koleva, D. Nikolova, D. Yankov, S. Danova^{, “}Growth parameters of probiotic strain Lactobacillus plantarum, isolated from traditional white cheese”. Biotechnology & Biotechnological Equipment, 2009, 23SE, 861-865
   
7. 
   D. Nikolova, Y. Evstatieva, R. Georgieva, S. Danova, V. Savov, S. Ilieva, P. Dalev, Molecular taxonomic characterisation of probiotic strain Lactobacillus sp. 50P1, 2009, 23SE, 779-782
   
8. 
   M. Petrova, R. Georgieva, S. Dimitonova, N. Ivanovska, N. Hadjieva and S. Danova, “Inhibitory activity of vaginal lactobacilli against human pathogens”, Biotechnology & Biotechnological Equipment, 2009, 23SE, 627-631
   
9. 
   P. Koleva, R. Georgieva, D. Nikolova, S. Danova, “Lactic acid microflora of Bulgarian milk products ^{Biotechnology & Biotechnological Equipment 2009, 23SE, 856-860}
   
10. 
    M. Petrova, R. Georgieva, L. Dojchinovska, N. Kirilov, I. Iliev, S. Antonova, N. Hadjieva, I. Ivanova, S. Danova, Lactic Acid Bacteria against pathogenic microbes. Tracia Journal of Sciences, 2009, 7 (2), 33-39
    
11. 
    G. Dobreva-Yosifova, L. Yocheva, A. Mehmed, S. Danova and S. Antonova-Nikolova. In vitro characterization of probiotic properties of lactic acid bacteria from Bulgarian rye sourdoughs Biotechnology & Biotechnological Equipment, 2009, 23SE, 801-805
    
12. 
    N.Kirilov, T. Petkova, J. Atanasova, Sv. Danova, I. Iliev, Y. Popov, T. Haertle and I. V. Ivanova. Proteolytic activity in lactic acid bacteria from iraq, armenia and bulgaria Biotechnology & Biotechnological Equipment, 2009, 23SE, 643-646
    
13. 
    S.P. Dimitonova, B.V. Bakalov, R.N. Aleksandrova-Georgieva, S.T. Danova, Phenotypic and molecular identification of vaginal Lactobacillus isolates from Bulgarian women. Journal of Microbiology, Immunology and Infection, 2008, 41, 469-477
    
14. 
    S. P. Stoylov, A. Gurova, R. Georgieva, S. Danova Do bacteria have an electric permanent dipole moment? Colloids and Surfaces B: Biointerfaces, 2008, 64, 255–259
    
15. 
    R. N. Georgieva, I. N. Iliev, V. A. Chipeva^,, S. P. Dimitonova, J. Samelis, S.T. Danova, Identification and in vitro characterisation of L. plantarum strains from artisanal Bulgarian white brined cheeses, Journal of Basic Microbiology, 2008, 48, 234-244
    
16. 
    Silviya P. Dimitonova, Boris V. Bakalov, Julia P. Serkedjieva and Svetla T. Danova. Inhibitory activity and protective properties of vaginal lactobacilli from healthy Bulgarian women. Anaerobe, 2007, 13 178–184
    
17. 
    Todorov S.D., Botes M., Danova S.T. and Dicks L.M.T. Probiotic properties of Lactococcus lactis subsp. lactis HV219, isolated from human vaginal secretions. Journal of Applied Microbiology, 2007, 103, 629-639
    
18. 
    Irena Tzvetkova, Michèle Dalgalarrondo, Svetla Danova, Ilia Iliev, Thomas Haertlé, Iskra Ivanova and Jean-Marc Chobert. Hydrolysis of major dairy proteins by lactic acid bacteria from Bulgarian yogurts. Journal of Food Biochemistry 2007, 31 (5), 680-702
    
19. 
    Galina D. Stoyancheva, Svetla T. Danova and Ivo Y. Boudakov. Molecular identification of vaginal lactobacilli isolated from Bulgarian women, Antonie van Leeuwenhoek International journal of general and molecular microbiology 2006, OCT 2006, 90 (3): 201-210
    
20. 
    Lidiya Angelova, Michèle Dalgalarrondo, Ignat Minkov, Svetla Danova, Nicolai Kirilov, Julia Serkedjieva, Jean-Marc Chobert, Thomas Haertlé, Iskra Ivanova. Purification and characterisation of a protease inhibitor from Streptomyces chromofuscus 34-1 with an antiviral activity. Biochimica et Biophysica Acta GS, 2006, AUG 1760 (8): 1210-1216
    
21. 
    Todorov Svetoslav D., Stoyancheva Galina D., Martina Meincken, Danova Svetla T. and Dicks Leon M.T. Characterization of bacteriocin HV219, produced by Lactococcus lactis subsp. lactis HV219 isolated from human vaginal secretions, Journal of Basic Microbiology 2006, Vol. 46, No. 3, 226-238
    
22. 
    S.T. Danova, G. Stoyancheva, Y. Manasiev and V. Miteva, Development and application of random DIG labelled probes based on total genomic Lactobacillus DNA”, World Journal of Microbiology & Biotechnology, 2005, 21: 835-842
    
23. 
    Svetla Danova, Kaloyan Petrov, Plamen Pavlov, Penka Petrova Isolation and characterization of Lactobacillus strains involved in koumiss fermentation”, International Journal of Dairy Technology, 2005, vol. 58, No 2 May, 100-105.