Резюмета на статии с приносни елементи

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Резюмета на статии с приносни елементи

на доц. д-р Димитър Диманов Димитров

Институт по тютюна и тютюневите изделия, ССА
Диманов Д., А. Атанасов, 1986. Разработване на регенеративна система от мезофилни протопласти при Nicotiana Tabacum / Virginia 89/ и Nicotiana alata. Генетика и селекция, кн. 5, 477-479.

Abstract. Mesophyll protoplasts of N. tabacum (cv. 89) and N. alata werw prodused from leaves, by degradation of the peptocellulose membrane with enzyme solution containing 0,3% Cellulase Onozuka R10 , 0,15% Driselase and 0,2% Macerozyme R10. The enzyme treatment lasted 15-17 h at temperature 22-26oC.

The cell colonies formed after 10-14 day long culturing in modified T0 and KA1 media were plated on hard medium for iduction of organogenesis. From the colonies plated on hard media in N. tabacum (cv. 89) after six weeks and N. alata after eight weeks developed regenerants, which were rooted on a B medium.

Диманов Д., А. Атанасов, Е. Цикова, 1986. Цитологические исследования протопластных культур Nicotiana Tabacum / Virginia 89/ и Nicotiana alata и их гибридных продуктов. Молодежная конференция по генетике, Варна, 12-17 сентебря, 156-159.

Abstract. Cytological analysis enabled the detection of initiation of division of parental protoplasts oridinating from N. alata and N. tabacum (V. 89) and somatic hybride products between them. The division of the two species was initiated 72 h after the isolation, mitosis being predominant in N. alata and N. tabacum exhibited predominant shoot formation. The products of hybridization, as determined by the difference in size of the nucleus present in them, started division on the 7-th day, the two parental nucleus undergo independent division.
Dimanov D, A. Atanasov, 1986. Somatic hybridization between Nicotiana tabacum and Nicotiana alata. VІ International congress of plant tissue and cell culture, August 3-8, University of Minnesota, Minneapolis, Minnesota, USA.

Abstract.The aim of this study is to develop a high efficiency system for isolation, cultivation and secondary regeneration in both N. tabacum and N. alata species in order to be used for somatic hybridization. The plating efficiency was 60-80 % in N. tabacum and 30-50% in N. alata on modified T0 media (Bourgin et al., 1978). The secondary bud formation is wide spread phenomenon in both species. For the elimination of the parent protoplasts treatment with Iodacetate ( 7.5 mM for 30 min and y- irradiation Co60 ) 20 kr were used. Morphological, cytological and biochemical investigations are in progress in order to examine the characters of the newly obtained colonies and plants after fusion.
Диманов Д., А. Атанасов, 1988. Проучване ефекта на някои ядрени и цитоплазмени инхибитори върху жизнеността на протопластни култури от Nicotiana alata и Nicotiana. Tabacum /Линия Виржиния 89/ с оглед осъществяване на соматична хибридизация между тях. Генетика и селекция, год. 21, № 6, 486-493. София.

Резюме. В процессе разработки эффектвной системы ранней селекции соматических гибридов и получения цибридов гибридов с асимметрическими ядрами испытывали влияние разных концентраций йодацетата /3, 5, 7.5, 10 mM при инкобировании в течение 4 и 20 min/ на развитие протопластов мезофилла N. Alata и N. Tabacum( линия Виржиния 89), а также влияние разных доз y- лучей (Co60) (77, 115, 154, и 192 Gy), которыми облучали протопласты и суспензии тех же видов. В качестве оптимальных параметров в экспериментах соматической гибридизации этих видов определены концентрация йодацетата 5 mM, применявшаяся в течение 4 minq и доза облучения y- лучами (Co60) 154 Gy. В таких условиях протопласты сохраняют жизнеспособность некоторое время, но утрачивают способность к делению. Доза порядка 115 Gy не препятствует образованию каллуса, но подавляет морфогенез. Облучение 77 Gy позволяет регенерацию 7-8% регенератов по отношению к контролюлю.
Dimanov D, 1989. Somatic hybridization between Nicotiana alata и Nicotiana. tabacum and intra-specific electrocell fusion in Solanum tuberosum; International course on advances and problems of plant cell and tissue culture held at Centro international de agriculture tropical CIAT Cali, April 23, May 6, Colombia.

Abstract. Somatic hybridization between N. alata and N. tabacum was used for transferring resistance from TSWV (tomato spotied wild virus) fro N. alata to N. tabacum.

For the elimination of the parent protoplasts treatment with Iodacetate (5 mM for 4 min and a gamma –ray Co 60 dose 154 Gy ) were used. At these conditions protrplasts remain alive for a certain period of time but loose their ability to divide. For the same purpose UV light was successfully used.

The mesophyll and respectively mesophyll and cell suspension protoplasts were fused by method of Menczel et al. (1981). The density of the mixed protoplasts suspension was 3-4. 105 pr/ml.

Citological analysis enabled the detection of somatic hybride products. The products of hybridization, as determined by the difference in size of the nucleus present in them, started division on the 7th day, the two parental nucleus undergo independent division. Newly obtained hybrid plants are still being tested to resistance for TSWV.

Mesophyll protoplasts from 20 S. tuberosum lines were fused by electrofusion. For obtaining the optimal conditions different parameters were investigated. The research was aided from cytological analysis of fusants.
Dimanov D, 1989. Determination the optimal variant of fusing to obtain maximal yield of fused products in protoplasts derived from Nicotiana alata and N. tabacum /Virginia89/, Fourth International Youth School – Conference of genetics, Albena, 19-23 Sept 1988,169-172, 1989. Sofia.

Summary: Three different methods for fusing of mesophil protoplasts derived from N.alata and N. tabacum (L. Virginia 89) have been tested. Variant III proved to be the most efficient, in which 25% of fused products were obtained. This result has been obtained at temperature 240C, which occurred the optimal for the vitability of the protoplasts. Besides, in this variant the fusing products remain free in nutrient medium, which makes taking probes for citologycal analysis easier and it is extremely important for the development of the fused products.
Диманов Д., А. Атанасов, 1989. Проучване на възможността за използване на УВ светлина в опити по соматична хибридизация при протопластни култури от Nicotiana alata и Nicotiana. Tabacum / Линия Виржиния 89 /, Генетика и селекция, год. 22, № 1, 40-44.

Summary: N. alata and N. tabacum (L. Virginia 89) protoplasts and suspensions were UV-irradiated (wave length 254 nm for 1, 3, 5 min at 40 and 200 mm distance between the UV source and protoplasts for the purpose of assessing the conditions leading to productior of cybrids and hybrids with asymmetric nuclei. As a result 50% protoplasts alive till the 5th day post UV irradiation (3 min, 200 mm distance) were found. These parameters of irradiation were accepted as optimal. N. alata protoplasts were fused with N. tabacum (L. Virginia 89) protoplasts in two treatments- processed and not processed. By modifying the method of cytological analysis the hybrid nature of the produced by fusion of UV-irradiated N. alata protoplasts and N. tabacum protoplasts treated with iodineacetate (L. Virginia 89) mixed products was registered. These heterokaryoms begin mitosis and start producing new colonies.

Диманов Д., А. Атанасов,1990. Изследване на възможността за съществуване на природна резистентност към антибиотици при Nicotiana alata и Nicotiana. Tabacum / Линия Виржиния 89 /, Генетика и селекция, год. 23, № 2, 130-136.

Summary: The possibility for existing of natural resistance to antibiotics in N.alata and N. tabacum (line Virginia 89) was investigated by elaborating a system for breeding somatic hybrids of the two species. Toleraance to various concentrations of: streptomycin-(100mg/l, 200 mg/l, 500 mg/l), kanamycin-(25 mg/l, 50 mg/l, 75 mg/l), chloramphenicol (5 mg/l, 20 mg/l, 50 mg/l), cycloheximid (0.1 mg/l, 1 mg/l, 5 mg/l) was tested on level of plants, cultivatedin test-tubes and on a levelof cell colonies of protoplasts, plated in an organogenesis medium, containing the respective antibiotics. The result of these studies was the assessment of natural resistance to 200 mg/l streptomycine in the species N. alata, which was not present in the species N. tabacum(L. Virginia 89). At this concentration of the antibiotic the number of green regenerants was very high (above 85% of the control) and, moreover, the plant,s development of the species, cultivated in test-tube, was not inhibited.
Диманов Д., Л. Влахова, Й. Бързакова, 1990. Калусогенез и регенериране на растения при домати. ІV-та младежка научно – практическа конференция „ Приносът на младежта за повишаване ефективността на растеноевъдството, Чирпан, 7-8 юли.

Резюме: Съдадена е ефективна система за регенериране на растения от морфогенетичен калус при три генотипа домати(Licopersicon esculentum Mill)-1104, P2046 и Monalbo. В резултат на голям брой експерименти /10/ е установено, че оптимална за индуциране на морфогенетичен калуспри трите генотипа домати е хранителна среда с 3 мг/л –НОК/нафтил оцетна киселина/ и 1 мг/л БАП /бензиламинопурин/. В хода на експериментите са наблюдавани сортови различия. Линии 1104 и Monalbo се характеризират с по-бързо и по-добро развитие на калусна тъкан в сравнение с Р2046. Подходяща за регенериране на растения при изпитваните генотипи домати е средар съдържаща 2 мг/л БАП. За увеличаване на процента на регенерираните растения от особено значение е периодичното субкултивиране /пасиране/ на калусната тъкан върху една и съща регенеративна среда. Процесът на морфогенезис силно зависи от изследвания генотип. Той започва най-рано и е с най-висок процент при линия 1104. Относително с по-нисък процент на регенерация, но напълно достатъчен за бъдещи ескперименти по клетъчна селекция се характеризира линия Р2046.
Диманов Д., Й. Бързакова, Л. Влахова, 1990. Соматичен ембриогенез и регенериране на растения при пшеница / Triticum aestivum L./. ІV-та младежка научно-практическа конференция „ Приносът на младежта за повишаване на ефективността на растениевъдството, Чирпан, 7-8 юли.

Резюме: Успешното приложение на растителните биотехнологии при пшеницата /T. аestivum L./ в най-голяма степен зависи от възможността за регенериране на растения от единични клетки. Индуцирането на пъпки в клетъчни и тъканни култури е критичен момент при реализирането на потенциалните възможности за регенерация.

В предложената работа са изследвани условията за получаване на ембриогенетичен калус и регенериране на растения от него при пет сорта зимна мека пшеница – Садово 1, Тунджа, Супер Х, К 609 и 65322 Си 14067. За изходен материал са използвани зрели и незрели зародиши от посочените сортове. Изпитани са 11 варианта хранителни среди за индуциране на калус, в резултат на което са установени три от тях, характеризиращи се с най-добър ефект. От особено значение за този процес е наличието в тези среди на 2,5 мг/л 2л4 Д, а така също на някои аминокиселини и абсцизиева киселина. Жизнеността на калусна тъкан от незрели зародиши бе проверена при продължително култивиране- 8 месеца. Два генотипа- Садово 1 и Тунджа показаха известно запазване на морфогенетичния потенциал на такава култура. Като най-подходяща за регенериране на растения от ембриогенетичен калус, получен от зрели и незрели зародиши е определена среда с 2 мг/л БАП /бензиламинопурин/. С най-висок процент на регенериране на растения от първичен калус се характеризират сортовете Тунджа и Садово 1. Не е без значение факта, че този процент е висок освен при незрелите зародеше/които са най-често използвани от изследователите/, но и при зрелите, които както е известно са много по-труден обект за еспериментиран

Dimanov D, Mölers K., 1990. Determination of some parameters for electrofusion of mesophillous protoplasts in potatoes, Fifth international youth conference on genetics, Albena, 11-15 Sept.182-185,Sofia.

Abstract: The purpose of the present work is to establich the duration of the direct current square- wave impuls in preliminary determined other factors an the % of electrofusion and yeld of binucleate products in potatoes( Solanum tuberosum L.).

The option of electrofusion in which the alternating current field with 10 v intensity, frequency of 1000 kH applied for 40-45 s. and a direct current impuls with 200 V and duration of 20ms. Appears to be optimal for us. In this case 26,5 % fused products of which 17,6 % binucleate cells were obtained. One can works with impulses of a range of 10 and 40 ms., but work with an impuls of 80 ms. Is not recommended although the fusion percentage is high. In this case not only the relative part of polycarions is increased, but the number of destructed cells is increased too.

By the parameters for electrofusion of mesopfyllous protoplasts in potato established by us, numerous experiments were carried out, and plants which are subject of analyses for proving their hybrid character were regenerated.
Димитрова Д., Д. Диманов, Р. Русева, 1992. Проучване влиянието на твърди и течни хранителни среди върху размножаването на картофи in vitro. Растениевъдни науки, год. ХХІХ, № 3-4, 68-72.

Abstract: The cultivars Bobur, Estima, Nela and Vivax have been cultivated on basic nutritive medium MS(1962) and B medium (Dimanov, Atanasov, 1986) with the aim of studing the effect of solid and fluid nutritive media on potato microreproduction. As a result of the trials it has been found that the nutritive medium B is most suitable for reproduction and rooting of the plants of the genotypes reported herein. It is of a particular importance to point out that the fluid variants of the MS and B media are more favourable for development of the potato cultivars used in the trial. Cultivar differences are manifested in the tested variants regarding the explant height and root-system development.
Atanasov A., D. Dimanov et al, 1991. Transfer of resistance to tomato spotted wilt virus /TSWV/ from wild Nicotiana species to Nicotiana tabacum via somatic hybridization, VІІІ International protoplast symposium Uppsala, Sweden, 16-20 June, Physiologia plantarum 82/1/

Abstract: Mesophyl protoplsts of IAA- treated N. tabacum and UV- irradiated N. alata were PEG-fused. The regenerants and their F1 and BC1 progenies were morphologically, biochemically, citologically, ELIZA and field tested. Studies on the fate of the TSWV-resistance in next progenies are in progress.

In order to establich a chromosome-specific isozime markers for N.alatas, TSWV- resistance, asymmetric somatic hybrids between N. plumbaginifolia/NR-/ mutant and –irradiated N. alata were obtained. Cytological, biochemical and ELIZA tests were carried out. The dictribution of the isozyme loci and the TSWV –resistance coding gene over the N. alata chromrsome set will be explained by the comparison of all analyses results.

To create an efficient system of somatic hybridization for transfer of TSWV- resistance in different commercial-grown Bulgarian tobacco cvs., transgenic Km-resistant N. alata and N. sanderae were obtained. The fusion experiments are in progress.
Sohou F., S. Petkova, D. Dimanov, 1994. Histological analysis of secondary induced embryogenesis in callus culture from mature rice embryos / Oryza sativa L. /. Biotechnology biotechnological equipment. 8/1994/1 p.41- 43.Sofia.

Abstract: Histological analysis gave evidence of the induction of embryogenesis from mature embryos in callus culture in two rice genotypes: cultivars (cvs.) Rosa and Belozeme, belonging to Oryza sativa, var. ital. The usual stage: globula, heart and torpedo and full embryoid with shoot and root and vascular tissues, typical for the normal process of embryogenesis were observed. The regenerated plants were were of embryoid origin, thus allowing to regenerate whole plants without the need of further root or shoot initiation.
Dimanov D., Petkova, D, Sobou F.,1994. Embryogenic calli induction and plant regeneration in wheat / Triticum sativum L./ and rice /Oyzisa sativa L./ under unified culture conditions. Biotechnology biotechnological equipment. 8/1994/1 p.44 – 49.,Sofia.

Abstract: A culture medium, indusing regenerative callus formation (medium 3) by cultures of mature and immature embryos of five wheat genotypes and mature embryos of two rice genotypes has been optimized.

An unified culture medium for plant regeneration from callus of bothwheat and rice species (medium 13) has been establiched.

An efficient system for regenerative callus induction and subsequent plant regeneration from immature and mature embryos of five wheat genotypes and mature embryos of two rice genotypes has been developed.
Диманов Д., Д. Димитрова., К. Върбанова 2001. Влияние на различен хранителен режим върху микроразмножаване на сортове хризантема. Конференция с международно участие “Екологични проблеми на земеделието”АГРОЕКО”,Науч. трудове, АУ, т. XLVI, кн.1, с.129- 134.

Abstract: The aim of the research was to establish the possibilities for in vitro micropropagation of tree chrysanthemum genotypes-Dark Westland, Refur, Alex Bedzer on two nutrient mediums-MS(6) and B (1)- in tree different forms each, namely: agar, liquid and liquid with perlite. The results obtained have proved the possibility of in vitro cultivation of the said Chrysanthemum cultivars on a liquid medium and on a liquid medium with perlite. The nutrient medium B has proved to be the most suitable for micropropagationof the Crysanthemumgenotypes studied. The importance of genotype in the processes of stem proliferation, leaf formation and rhizogenesis was confirmed.
Диманов Д., П. Кировски, В. Ройчев, 2001. Влияние на различен хранителен режим при in vitro култивиране на лоза. Аграрен Университет – Пловдив, Юбилейна научна сесия „ 80 години висше агрономическо образование в България”. Научни трудове, том ХLVІ, кн. 5, 251-256.

Abstract: It has been researched the possibility for in vitro cultivation at a different nutritive regime of microcuttings from seedless wines cv. Rusalka 3, Korint black, the seeded cv. Caberne Souvignon and the rootstock Berlandieri x Riparia SO4. It has been established that for inducement of root formation medium B is most appropriate.

Over the nutritive mediums MS and B green vine explants may be successfully grown, as there a specific reaction of researcher cultivars in the dynamics of growth, leaf number and length of the shoots.

Dimitrova D., D. Dimanov, 2002. In vitro micropropagation of potatoes.

Effect of different nutritive conditions. ІV Haционална научно-техническа конференция с международно участие „Екология и здраве”, Сборник с доклади, с.141-146.

Abstract:The scope of this study is to explore the micro-propagation of potato cultivars: Ponto, Ropta, Maranka and Alina, which are in vitro cultivated in both media: MS and B. Medium B is examined in three variants: agar, liquid and liquid with PerliteR. The results prove the priority of medium B (its three variants) over medium MS when in vitro propagation with the reported genotypes is carried out.

The needed conditions for in vitro explants implantation are met for the examined genotypes when both variants: liquid medium B and liquid medium with PerliteR are used. The positive effect of medium B on the proliferation of stems as well lives formation for four potato cultivars is clearly demonstrated. The specific reaction of a genotype in the processes of rooting and proliferation during in vitro tested variants of nutritive media is confirmed.

Ройчев В., П. Ботянски, С. Янчева, Д. Диманов, 2002. Влияние на хранителната среда и произхода на лозови растения, отглеждани при in vitro условия върху динамиката в нарастването на вегетативните им органи. Юбилейна научна сесия с международно участие. 100 години Институт по лозарство и винарство, Плевен – 2002, SPS PRINT, София, 187-194.

The influence of the culture medium and the origin of in vitro grape plants on the dynamics of vegetative growth has been investigated. Onebud micro cuttings obtained from zygotic and somatic embryos of inbreeding ovules of seedless cultivar Russalka 3 have been used as experimental material. It was established that the dynamics of development of the plants from both groups and culture media has two periods of slow and intensive shoot diameter, number and length of the roots was normal without significaht deviations. Both nutrition media- VM and B were suitable for in vitro growth of grape micro cuttings, however the first medium had better effect on the intensity and development of the plants. According to the explant origin a tendency was observed for the green microcuttings from zygotic embryos to demonstrate faster growth and development of bigger vegetative organs than somatic embryos. There was statistically established specificity in the dependence of the growth.

Диманов Д., П. Запрянова, 2002. Влияние на агроекологичните условия върху добива и качеството на различни генотипи от произход Неврокоп, Сборник доклади от Втора Балканска научна конференция „Качество и ефективност на производството, обработката и преработката на тютюна,214-217, Пловдив.

Abstract: The effect of agroecological conditions on the yield and quality of tobacco genotypes of the “Nevrocop” region was studied for two years in two different regions- the Experimental field in the village of Markovo and the Tobacco Experimental Station in Gotse Delchev. The results obtained show that the tobacco from the Gotse Delchev region is characterized by a higher stability of yield, quality and gross production per decare. The yield obtained at Gotse Delchev was much higher than in Markovo. The different agroecological conditions of our experiments show the great significance of genotypes- the performance of Line 1 was the weakest in both locations, while in most cases Line 2 came up as more promising. The appropriate agroecological conditions are a prerequisite for the realization of all the potentialities of tobacco genotypes.
Dimanov D., 2002. Somaclonal variation in tobacco progenies derived from a simple F2 hybrid, Tobacco, vol. 52, №3-4, 72-77.

ABSTRACT. An analysis was carried out of the variation coefficients of some characters in the progenies of callus culture regenerants, induced by somatic tissue from a single plant derived from a simple F2 tobacco hybrid. It was found that by their variability the progenies tested are homogeneous with some minor exceptions, which shows that the genetic processes influencing variability are identical. In some characters and progenies the variances fluctuate within wide limits, but after their elimination, the variability by the Cochran and Bartlet criteria in the remaining progenies is homo-geneous. In parallel with the genetic processes in the different progenies and hybrids in F3, some deviations are evidenced in individual characters and progenies that are reflected mainly by the variances and the variation coefficients. These changes are most likely due to somaclonal variation.

Key words: criteria of variability, Oriental tobacco, somaclonal variation.

Summary: The results obtained from the study of somaclonal progenies of a tobacco hybrid combination in F2 were analysed.As a result of genetic and somaclonal variation the progenies obtained differ from one another in the characters studied. These differences are well pronounced and provide good possibilities for further selection of individual

lines.Regardless of its type, the genetic variance is correctly expressed by the coefficients of hereditability which are which were proved to be highly reliable. This especially well manifested with respect to plant height.The phenotypic correlation and regression dependencies between the characters studied are positive and highly reliable and are conditioned by the ecological dependencies as well. The genotypic correlations and regressions are also positive and strongly manifested , but are not efficiently reliable with respect to plant height:leaf number and plant height:leaf length, whreas with respect to leaf number:leaf length they are 2-nd rank reliable.

Key words: hereditability, Oriental tobacco, somaclonal variation..
Диманов Д., 2005. Произход Джебел – състояние и перспектива, Български тютюн, № 2, 14-19.

Резюме: Непосредствено със създаването на опитното поле по тютюна в Джебел през 1929 година се поставя и задачата за сортово проучване и типизиране на тютюн Джебел басма. В унисон с изискванията на Европейския съюз и фирмите производителки на цигари и тютюневи изделия е необходимо възстановяване на доброто търговско име и пушателни качества на произход “Джебел басма”. Това налага обновяване на сортовия състав на произхода със сортове отличаващи се с високо качество, вкус и аромат характерни за класическата басма, със следните показатели: средна височина на растенията 80 – 100 см, със среден брой листа 28 – 32 бр. Листата да бъдат дребни, овално елипсовидни с накъдрена петура и размери до и под 14 см. на тези от средния пояс, с фина нерватура. Изсушените листа са съдържателни, до средно съдържателни, нежна структура, тънък главен нерв – среден процент – 19 %, проявена еластичност, изразен гланц и “изпеченост”, цвят – жълто-оранжев, оранжев, оранжевочервен за висококачествените фракции.
Даньо С., Д. Диманов, 2006.Цвят при ориенталски тютюни сортова група Джебел басма в зависимост от характеристиката на полифенолите: химиметричен подход, Български тютюн, 5, 14-21.

Summary:The influence of polyphenols of different Djebel basma tobacco genotypes on the color of dry tobacco was studied by applying a chemometric approach. The cultivar Djebel 576 was used as a standard. High performance liquid chromatography (HPLC) was applied to obtain primary data on the polyphenol pattern. The color estimation was done by using a regression model based on the polyphenol data and compared to the sensory evaluation. The chromatographic data were processed by means of pattern recognition methods (PRM), and the similarity indexes (Is, %) between the introduced cultivars, as well as between them and the standard, were calculated. All tobacco genotypes differ in the polyphenol pattern in relation to the standard and toward them. The data point that the color of dry tobacco is determined by the genotype-specific composition of the polyphenol complex. The chemometric evaluation predicts the improved color of Line 3 with respect to the standard Djebel 576 and the other genotypes.
Даньо С, Д. Диманов, 2006. Аромат при ориенталски тютюни сортова група Джебел басма в зависимост от характеристиката на валериановите киселини: химиметричен подход, Български тютюн, №6, 15-21.

Summary:The influence of valeric acids characteristics of different Djebel basma tobacco genotypes on the aroma of tobacco smoke was studied by applying a chemometric approach. The cultivar Djebel 576 was used as a standard. Capillary gas chromatography (CGC) was applied to obtain primary data on the valeric acids. The aroma of tobacco smoke was evaluated by regression model involving isovaleric acid content. The data point that the aroma of tobacco smoke is determined by the genotype-specific composition of the valeric acids. The chemometric evaluation predicts the improved smoke aroma of Line 3 with respect to the standard Djebel 576 and the other genotypes
Dagnon S, D. Dimanov, 2007. Chemometric Evaluation of the Colour and Smoke Aroma in Oriental Tobaccos Based on the Poliphenol and Valeric Acid Cultivar Characteristics as Influenced by the Genotype, Bulgarian Journal of Agricultural Science, 13, 459-466.

Abstract: The influence of polyphenols and valeric acids of Oriental tobacco cultivars on the colour of dry tobacco and the aroma of tobacco smoke was studied by applying a chemometric approach. A group of Djebel basma genotypes and a local cultivar Djebel 576 used as a standard were involved in the study. To obtain primary data on the polyphenol pattern related to colour estimation high performance liquid chromatography (HPLC) was used, whereas capillary gas chromatography (CGC) was applied to get information on valeric acids content, related to aroma characteristics. Regression models involving the polyphenol data and the isovaleric acid content were used for colour and aroma estimation, respectively the HPLC data were processed by means of pattern recognition methods (PRM), and the similarity indexes (Is, %) between the selection lines, as well as between them and the standard, were calculated. It was established that all cultivars are distinct from the standard in the polyphenol pattern. The data point that the colour of dry tobacco and the aroma of tobacco smoke are determined by the genotype-specific composition of the polyphenol complex and of the valeric acids, varying in the different cultivars. The chemometric evaluation of tobacco colour and smoke aroma allows predicting the improved quality characteristics of the selection line L 3 with respect to the standard Djebel 576 and the other genotypes.

Key words: Oriental tobaccos, colour, smoke aroma, polyphenols, valeric acids, chemometrics
Диманов Д. 2007. Сортове Мюмюново семе и Секирка. Български тютюн, 5, 12-14.

Резюме:В миналото типичен представител на джебелските сортове е била Джебелската местна ( хас ) басма разпространена в целия район и дори в малки количества в някои башибалийски под райони.В резултат на кръстосаното опрашване на отделни форми, продължителен отбор провеждан от тютюнопроизводителите и под влияние на специфичните екологични условия в Джебелската тютюнева област се съэдава голямо разнообразие от местни тютюневи сортове от типичната “хас Джебел басма”. Тези сортове се различават помежду си по редица морфологични, биологични и технологични белези.
Диманов Д., 2007. Технологии за отглеждане на ориенталски тютюн произход „ Джебел”, Български тютюн, бр. 4, 11-13.

Резюме: Целта на настоящата публикация е да се представи възможно в най-сбит вид технология за производство на тютюн от екотип „Джебел басма, като е акцентувано на най-важните моменти от производсвения цикъл. Тютюните от екотип „Джебел басма” са широко известни на световните пазари. Запазването на доброто търговско име на тези тютюни, а така също и повишаването на търсенето им е от голямо значение за българското тютюнево стопанство. Ето защо правилният подбор на сортове и стриктното спазване на технологията по отглеждането им е от първостепенно значение за получаване на качествена суровина, удовлетворяваща в най-висока степен производители и търговци.
Dimanov D., R. Todorova 2009. New varieties of Dzhebel basma, Genetics and Breeding, vol. 38, №3-4, 123-127.

Abstract: In agreement with the European Union and Tobacco trading and cigarettes companies requirements a recreation of the food trade and commercial properties of the smoking qualities of ecotype “Dzebel basma” is necessary Dzebel basma variety structure has to be renovated through development of high quality, flavour and aroma new genotypes, typical for classical (has)basma. To achieve the above purpose, two new varieties of oriental tobacco ecotype “Dzebel basma” have been developed in 2008. For the varieties Dzebel basma 1 and Dzebel basma 2 we got certificates. The genotypes submitted here are true (has) basmas. Their biological, morphological, economic and technological indices combine the qualities of the Dzebel tobacco with new market requirements.

Key words: Dzebel basma 1, Dzebel basma 2, has basma, ecotype
Диманов Д., Ат. Димитров, 2004. Използване на биотехнологични методи за борба с вредителите по тютюна, 60 години ИТТИ, Юбилейна научна конференция с международно участие, Сборник доклади – 3-5 ноември,276-281, Пловдив.

Abstract: This article specifies the advantages of tissue cultures, including callus tissues , suspended cell cultures and isolation of cells and protoplasts. Tissue cultures provide useful information for studying the resistance of plants to the major diseases of tobacco. The system of tissue cultures that is carefully determined and provides the best proof of the applicability of these techniques in studying the internal parasitic effects is tobacco tissue cultures (Nicotiana tabacum L. ). The resistance of tobacco callus cultures is due to several factors, namely: temperature, inoculum concentration, callus morphology and especially the balance of phytohormones in the growth medium. A description is presented of the characteristics of resistant, reacting callus tissues. The estimation of disease resistance is based on varies factors: callus tissue type, composition of the nutrient medium, genetically identical pathogen isolates, inoculum and abiotic factors temperature and light.
Друмева – Йончева М., Д. Диманов, П. Запрянова, 2009. Хетерозисни прояви при хибридни линии тютюн тип Виржиния по отношение на някои основни биометрични показатели. Девета научно-практическа конференция с международно участие „ Екология, земеделие, животновъдство”, 14.05.2009, Пловдив, АГРОЕКО, Науч. Трудове, АУ, т.LІV, 113-118.

Abstract:The study carried out in 2006-2007 at the field of the Tobacco and Tobacco Products Institute- Markovo. Four introduced varieties of Virginia tobacco and their two F1 hybrids were investigated for the following characters: plant height, length and width of middle leaf. The aim of investigation was to study the heterosis effect for the above mentioned characters in progeny of F1 generation and to estimate the inheritance of the major biometric indexes, determining the yield. Overdominant inheritance was established of the features height of the plant and the width and the length of the 14 leaf in K326 x Л825. The higest heterosis effect compared to the better parent was established regarding the length and width of 14 leaf in both hybrids.
Диманов, Д., Р. Тодорова, Й. Кочев, 2010. Джебел басма 1- нов перспективен сорт истинска/хас/ „Джебел басма”. Научни трудове т. ІV част ІІ,, 168-172, Кърджали.

Резюме: С помощта на класическите селекционни методи е създаден нов сорт тютюн за Джебелска тютюнева област –Басма 1, който е високодобивен, с добра химико-технологична и дегустационна оценка.Сорт Джебел басма 1 има добре балансиран химичен състав за произхода. Съдържание на никотин /за трите години на изпитване 1999-2001 г./ по данни на ИАСАС – 2,01%, съдържание на разтворими въглехидрати – 14,60%, съдържание на азот – 2,02%.

По наши данни и данни от изпитванията на ИАСАС добивите са от порядъка на 170-200 кг/дка, в зависимост от агроклиматичните условия и стриктно спазване на агротехниката.

Диманов, Д., Р. Тодорова, Й. Кочев, 2010. Нов сорт Джебел басма 2 от екотип „Джебел басма”. Научни трудове т. ІV част ІІ, 173-177, Кърджали.

Резюме: С помощта на класическите селекционни методи е създаден нов сорт тютюн за Джебелска тютюнева област –Басма 2, който е високодобивен, с добра химико-технологична и дегустационна оценка.

Химичните показатели получени след изпитване на сорта в системата на ИАСАС и нашите данни, напълно удовлетворяват /висок никотин и балансирани захари/ съвременните търговски изисквания за екотип Джебел басма.
Диманов, Д., Р. Тодорова, Й. Кочев, 2010. Басма 13- нов ориенталски сорт тютюн. Научни трудове т. ІV част ІІ, 178-181, Кърджали.

Резюме: С помощта на класическите селекционни методи е създаден нов сорт тютюн за Джебелска тютюнева област –Басма 13, който е високодобивен, с добра химико-технологична и дегустационна оценка. Химичните показатели получени след изпитване на сорта в системата на ИАСАС и нашите данни, напълно удовлетворяват /висок никотин и балансирани захари/ съвременните търговски изисквания за екотип Джебел басма. Посочените резултати показват преимуществата на новият сорт, както по добив , така и по качество, спрямо стандарта – Джебел басма 576

Диманов Д., В.Машева, 2011. Нови сортове ориенталски тютюни от сортова група Басми. Български тютюн, 6. 23-27

Резюме: Тютюнът като стока за реализация се приема основно в зависимост от нейните потребителски качества. За разлика от другите селскостопански култури тютюневите сортове са изключително свързани с условията на отглеждане. Основни фактори за формиране на еднородността на тютюневите партиди са климатът с неговите компоненти- температура, валежи, относителна влажност и ветрове; почвата с ораграфските си особености – хумусно съдържание, механичен състав, воден капацитет; релефът с надморската си височина и вертикално разположение и сортът с биологичните си качества и специфични пушателно-вкусови свойства.

За това трябва да се увеличи сортовото разнообразие и съобразявайки се със средата на отглеждане, да се създадат на сорта оптимални условия за проявата на генетичния му потенциал.

В отдел „Селекция и семепроизводство” на Институт по тютюна и тютюневите изделия се работи за създаване на нови сортове и от трите сортови групи – Ориенталски, Виржиния и Бърлей. Тук представяме новите сортове ориенталски тютюн сортова група Басми, селекционирани в ИТТИ, Марково през последните години – Мюмюново семе, Секирка, Джебел Басма 1, Джебел Басма 2, Басма 13, Пловдив 380, Устина 54, Козарско 339 и Средногорска яка.
Dimanov, D. 2011.Djebel Basma – a New Generation of Oriental Tobacco Ecotype, Тутун/ Tobacco,Vol. 61, 7-12, 130-133.

Abstract: In compliance with tobacco trading and the requirements of cigarette companies, it is necessary to restore the good trade image and commercial properties of the smoking qualities of the ecotype Dzebel basma. The variety structure of Dzebel basma has to be renovated with new genotypes of high quality, flavour and aroma, typical for traditional basma.

In accordance to that, three varieties of oriental tobacco Dzebel basma – Dzebel basma 1, Dzebel basma 2, Basma 13 werw created and acknowledged by IASAS and Patent office of Bulgaria ( 2008-2010).

The genotypes presented here are genuine basma tobaccos. Their biological, economic and technological characteristics combine the qualities of the old Dzebel tobacco with new market requirements.

Key words: dzebel basma 1, dzebel basma 2, basma 13, oriental tobacco
Диманов Д., Д. Витанова, 2011.Проучване възможността за отглуждане на чужди ориенталски сортове при агроекологичните условия на район Неврокоп. Български Тютюн,бр.1,5-8.

Резюме: Проучени са биологичните и стопански качества на два сорта Катерини-Катерини 53 и Катерини М, при агроекологичните условия на район Неврокоп. Опитът е изведен в Опитното поле на Опитна станция по тютюна в гр. Г. Делчев. Въпреки, че е едногодишно, проучването е детайлно и показва, че гръцките сортове за реколта 2010 г. Отстъпват по стопански показатели и устойчивост на важните за района болести. Това според нас ги прави неподходящи за отглеждане в район Неврокоп. Това заключение се налага и от предишни наши наблюдения, които показват сходна тенденция.

Сортовете Катерини 53 и Катерини М са са ценни за селекцията за създаване на хибридни сортове с добри пушателни качества, търсени от търговските фирми.

Диманов Д.,2011. Състояние и насоки в селекцията на тютюна в България. Български Тютюн,бр.6, 13-17.

Резюме: На основата на досега извършената селекционна работа се очаква да бъдат селекционирани линии /евентуално соротове/, напълно удовлетворяващи пазарните изисквания и високо конкурентни на чуждата инвазия.

Създаване и поддържане на сортове тютюн, толерантни на абиотичен и биотичен стрес, формиращи стабилни добиви и рентабилно производство, позволяващи оптимизиране на сортовата структура на ориенталските и едролистните тютюни.

Masheva V., R.Todorova, D.Dimanov, 2009. Mode of Gene Action of Some Traits in Inter-Varietal Crosses of Oriental Tobacco (N.tabacum, L.). Genetics and Breeding,vol.38, № 3-4, 129-134

Abstract: The gene effects for plant height, number of leaves per plant, leaf length and breadth and flowering period of tobacco were studied using two crosses (Krumovgrad 988 Basma Xanthi 101) and (Harmanly 134 Basma Xanthi 101). The varieties used were divergent for all the characters. Additive component (d) was significant for leaf length and width, and flowering period in cross (Krumovgrad 988 Basma Xanthi 101) and length of 14th leaf in the other cross (Harmanly Basma 134 Basma Xanthi 101). Additive and non additive gene effects were significant for plant height and number of leaves in both crosses, and length of 21st leaf, leaf breadth and flowering period in (Harmanly Basma 134Basma Xanti101). In cross (Harmanly 134 Basma Xanthi 101) the presence of additive and non additive gene effects suggested the feasibility to evolve homozygous elite genotypes by cyclic method and inter se crossing among the desirable recombinants keeping adequate population size.

In cross (Krumovgrad 988 Basma Xanthi 101) additive effects were predominant in the expression of length and width of 14th and 21st leaf and flowering period and suggests that it be easier to isolate desired tobacco genotypes from this population by Pedigree method of selection. Involvement additive as well as epistatic (additive additive and additive dominance) effects of plant height and number of leaves to conserve additive as well as non-additive gene effects - cyclic method of breeding would be more effective.

Диманов Д., В.Машева, Р.Тодорова, А.Янчева, 2012. Сравнително изпитване на сортове тютюн от екотип Крумовград. Научна конференция с международно участие „Екология и здраве”.

Summary: It has been a comprehensive assessment of biological and economic performance of seven varieties of oriental tobacco ekotype Krumovgrad in agro-environmental conditions in the TTPI experimental field. Morphological and biological characteristics of all tested varieties fit to typical ecotipe Krumovgrad. Varieties Krumovgrad 78C, Krumovgrad 17 and Krumovgrad 944 are with higher productive potential yield and quality of dry tobacco than the standard variety Krumovgrad 988.
Dimanov D., Y. DyulgerskI, I. Zheliazkov, 2012. SELECTION OF BIG-LEAFS TOBACCOS IN BULGARIA – STATUS AND PERSPECTIVES, сп. Аграрни науки

Abstract: Big-leafs tobaccos take up a significant part of total tobacco production in Bulgaria. In our country they are presented with Virginia and Burley varieties groups. Yield and quality in our production seriously inferior to that in major producing countries. To maintain their production have to create and introduce new higher-quality and high-yield Virginia and Burley tobacco varieties.
DYULGERSKI Y., D. DIMANOV, IANTCEVA A., 2012. STUDY OPPORTUNITIES FOR USE OF HETEROSIS AND TRANSGRESSION TO OPTIMIZE LENGTH OF VEGETATION PERIOD IN BURLEY TOBACCO VARIETIES GROUP. ІХ Национална научно-техническа конференция с международно участие ”Екология и здраве”, Сборник с доклади, 131-134.

Abstract: The survey data show that the heterosis effect, albeit less pronounced there economic significance in terms of research indicator, while transgression.and selection process, has a limited importance in the breeding of Burley tobacco, in the length of vegetation period. Hybrid 1560 is a variant with the highest selection values, concerning studies indicator, because it is the highest values of both hypothetical and real heterosis effect.

Key words: Burley tobacco, heterosis, transgression, vegetative period


Abstract: We have researched the heterosis behaviour with regards to the height of the plants and the number of the leaves indexes. For this purpose, we have studied the Р1, Р2 and F1 populations of ten cross-fertilizations where local and introduced varieties of Burley tobacco participate. The data received shows that the heterosis effect is better pronounced with regards to the number of the leaves. Hybrid 1558 shows highest values of pronounced hypothetic and real heterosis with regards to both indexes. The heterosis effect is positively influenced by the distance of the parents used for hybridization.
Dyulgerski Y, Dimanov D., 2012. Study on Hetetozis Behaviour Related to the Leaves Size by the Tobacco of Burley Variety Group. Jour. of Acta agriculture Serbica( in pres).

Abstract: The study has been performed on the heterosis behaviour related to the following indications: length and width of the 7-8 leaf and length and width of 13-14 leaf. For this purpose, the research was focused on the populations of Р1, Р2 and F1 of ten cross-fertilizations where local and introduced varieties of Burley tobacco participate. The results show that the hetorosis effect could be best seen in the width of the 7-8 leaf and therefore it has an economical importance with regards to this index namely. The heterosis referring to the width of the leaves is more pronounced than their length. Also, with regards to the size of the leaves, the heterozis is better seen in the lower than in the upper pick zone (harvesting stalk position).
Djulgerski Y. D. Dimanov 2012. INHERITANCE OF THE NICOTINE IN BURLEY TOBACCO CROSSES. Tobacco, Vol. 62, № 1-6, 9-12.

Abstract: Examined the degree of dominance, heterosis and heritability of nicotine in tobacco cross hybrids. For this purpose, were studied populations P1, P2, F1 and F2 crosses of ten featuring local and introduced varieties of Burley tobacco. As a result of the analysis established a positive heterosis for economic value in most of the crosses tested. The most pronounced when he was in Hybrid 1457 and Hybrid 1462. Inheritance of nicotine overdominantly is incompletely dominant or additive. The direction of inheritance vary both in direction of the parent with the lower and the parent with higher levels of nicotine. In most of the studied hybrid combinations is set relatively high coefficient of heritability. So the selection with good quality of the seven genotypes will be more effective in earlier hybrid generations (F2 – F3). Hybrids 1466, 1472 and 1478, where they found low values ​​of heritability coefficient selection for nicotine will be effective in the later hybrid generations (F5–F6).

Dyulgerski Y , D. Dimanov, 2012. INHERITANCE OF THE LEAVES SIZE IN VIRGINIA TOBACCO CROSSES. Tobacco(in pres).

Abstract:Were studied succession, inheritance, the coefficient of the team and the number of genes acts of transgression and heterosis in terms of length and width of leaves from the middle harvesting zone in Virginia tobacco. For this purpose, populations were investigated to P1, P2, F1 and F2 of six hybrid combinations. Data from hybridological analysis showed that the samples studied by us Virginia tobacco, inheritance of the length and width of leaves of middle harvesting zone is overdominant and always in the direction of the parent with the higher values. Heterosis and transgression appear reliable means of increasing the width of the leaves from the middle harvesting zone in Virginia tobacco. Middle and high values are determine​​for inheritability, so the selection of this sign will be effective in the early generations.

The character and extent of the genetic interactions were determined by applying hybridological analysis as well as by the number of genes differentiating between the initial parent forms and expressions of heterosis and transgression referring to the feature size of the leaves, the objective being the selection of desired genotypes in six hybrid Virginia tobacco populations, involving introduced varieties.


Abstract: The posibility of morfogenetic calli from 3 tomatoes genotypes resistance to cultural filtrate of two race fusarium has been explored. In the experiment the food medium inducjing morfogenetic calli has been optimised in the 3 genoms of tomatoes containing 3 -NAA mg/l and 1 BAP mg/l. A Medium of 2 BAP mg/l is switable for regenerating the tested genotipes tomatoes, the percentage of the regenerated plants should be incredsed with every tissue transfer onto this medium. It has been found out that there is a strong dependence between morfogenesis and genotype. Line 1104 have the highest regenerating persentage. The 3 genotypes tomatoes have greater sensibility to fusarium - race 2. Line 1104 has the greatest stability to fusarium - race 1, while line P.2046 hds the strongest stability to fusarium - race 2.
Мутафчиева М., Д. Диманов, 2012. Биологични и стопански качества на интродуцирани мъжкостерилни линии тютюн от тип Бърлей, Международна конференция „Новости в аграрната наука за ефективно земеделие”.Земеделски институт, 27 – 28.09. 2012 г. Шумен, Сп. Растениевъдни науки /под печат.

Abstract: The study was cnducted in the period 2008-2010 in Agricultural Experimental Facility Khan Khrum, Shumen municipality. The study covered the biological and commercial properties of five introduced male- sterile lines of Burley tobacco. The Pliska 2002 variety was usd as a standard. All male-sterile lines are superior to the standard on the properties: plant height, number of leaves and size of the leaves of the middle ring (14th leaf). The duration of the vegetation period is 6 to 15 days longer than the standard. In terms of dry tobacco yield, the tested varieties exceed the standard Pliska 2002 with an average of 87-141 kg/acre (37.5-60.5%) dry tobacco.

Key words: tobacco, cytoplasmic male sterility, biological property
ДЮЛГЕРСКИ Й., Д. Диманов, 2012.ПРОУЧВАНЕ НА УНАСЛЕДЯВАНЕТО НА ВИСОЧИНАТА НА РАСТЕНИЯТА ПРИ КРЪСТОСКИ ТЮТЮН СОРТОВА ГРУПА ВИРЖИНИЯ. ІХ Национална научно-техническа конференция с международно участие”Екология и здраве”, Сборник с доклади, 329-332.

Резюме:Проучени са популациите на Р1, Р2, F1 и F2 на шест хибридни комбинации при тютюни от сортова група Виржиния. Данните от хибриологичния анализ показват, че унаследяването на височината на растенията е свръхдоминантното, с голямо влияние на епистазните взаймодействия. Установени са значими стойности по отношение на наследяемоста, поради което отбора по този признак ще бъде ефективен още в ранните генерации.

Abstract: The character and extent of the genetic interactions were determined by applying hybridological analysis as well as by the number of genes differentiating between the initial parent forms and expressions of heterosis and transgression referring to the feature height of plants, the objective being the selection of desired genotypes in six hybrid Burley tobacco populations, involving indigenous and introduced varieties. The results of the survey of Р1, Р2, F1 and F2 proved that the feature height of plants in Burley tobacco crosses is inherited overdominantly, the parent with higher values dominating. The environment affects is highly on the expression of the feature. The established low coefficients for inheritance of height of plants and effectiveness of selection by phenotype showed that the selection of genotypes characterized by a greater would prove to be more effective in the later hybrid generations.

Diulgerski Y., D. Dimova, D. Dimanov, 2012.EVALUATION OF QUALITY AND STABILITY OF BURLEY TOBACCO SAMPLES, сп. Растениевъдни науки/под печат/.

Abstract:Investigated and evaluated the quality and stability are fifteen promising genotypes using parameter Kang - Yield Stability, expressed by the percentage of first, second and third class. The analysis shows that the most favorable indicators is Burley variety 1344. Very good from a business standpoint are the results of Line 1390. These variations are weakly influenced by weather conditions over the years and have a stable economic indicators. Relatively good and valuable features are formed in the first-generation hybrids, but with poor were introduced varieties, especially the variety Tennessee 90, which in all tested variants is most sensitive to environmental conditions.

Summary:It has been a comprehensive assessment of biological and economic properties of five varieties of oriental tobacco ekotyp Krumovgrad in agro-environmental conditions of the experimental field-town of G. Delchev / region Nevrokop / .All tested varieties in their morphological and biological characteristics fit the typical variety of Krumovgrad .The varieties ekotyp Krumovgrad - Krumovgrad 78C and 68M are higher productive potential yield and quality of dried tobacco and excel tested varieties ekotyp Krumovgrad. From a general assessment made can be predicted that the investigated varieties can be grown not only in regions that were selected, but also in other similar soil and climatic characteristics of their regions.
DIMANOV D., V.MASHEVA, D. DIMITROVA, 2012. Effect of nutritive media on Solanaceae sp. Reproduction in vitro, Bulgarian Journal of Agricultural Science, (in pres).

Abstract: It has been researched the possibility for in vitro N.tabacum sp, S.tuberosum sp, S.lycopersicum sp. cultivars at a different nutritive mediums. A positive effect of liquid nutrient media on micropropagation in vitro in three Solanaceae species, compared to solid agar medium was established. Best for micropropagation in vitro in the varieties of three species - N. tabacum, S. tuberosum and S. lycopersicum is nutritive medium B. The importance of genotypein the reproduction in vitro has been demonstrate.The unified systeme for micropropagation in vitro – based on nutritive medium B of varieties of three family Solanaceae species was created.

Key words: nutritive media, in vitro reproduction, cultivars,
M. Docheva M., S. Dagnon2, St. Statkova, D. Dimanov,2012. Isolation of bioflavonoids from tobacco, Trakia Journal of Sciences(in press).


PURPOSE: The interest in bioflavonoids as antioxidants has increased remarkably over the last decade because of their protective effect against different diseases, including cardiovascular, inflammatory and neurological diseases, as well as cancers. However, the necessity of identifying a better cheaper commercial source of these medicinally important compounds is still valid. In this study an approach of extraction and isolation of flavonoids from Oriental tobaccos was investigated. It aimed to obtain extracts enriched in the flavonoid glycosides rutin and kaempferol-3- rutinoside. METHODS: Selective ultrasound-assisted extraction with mixture of methanol and ethyl acetate was used to extract the flavonoids from tobacco. Column chromatography on silica gel was employed to rutin and kaempferol-3-rutinoside purification by using ethyl acetate: methanol (7:3) as mobile phase. The quantitative determination of the polyphenols in tobacco and in the purified fractions was achieved by using HPLC-DAD. RESULTS: The obtained ethyl acetate/methanol fractions contained approximately 3.9 mg/g flavonoids. The purification procedure characterized with recovery 47% for rutin and kaempferol-3-rutinoside. The enriched fractions of flavonoids contained only traces of phenolic acids and nicotine. CONCLUSION: The results from this research indicated that tobacco can be regarded as a source for isolation of the bioflavonoids rutin and kaempferol-3-rutinoside. Thus, it seems tobacco can be utilized to obtain these medicinally important compounds.

Key words: rutin, kaempferol-3- rutinoside, purification, HPLC

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