Списък публикации Доц. Николай К. Христов



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      Патенти и сертификати:

1) Патент №: JP 2007-49922A 2007.3.1

Inventors: Ryozo Imai, Nikolai Kirilov Christov and Petya Koeva Christova

Title: New antibacterial protein and its use thereof


2) Сертификат №: 10855 ЦАРЕВИЦА СОРТ ИНБРЕДНА ЛИНИЯ АНИС 104ИР

ZEA MAYS (L.) INBREDNA LINIIA ANIS 104 IR

Собственици: АГРОБИОИНСТИТУТ (АБИ) КОСТИНБРОД; АНИС ООД, ПЛЕВЕН

Автори: Христов, Николай К.; Христов, Кирил Н.; Атанасов, Атанас И.; Христова, Пенка И.; Влахов, Сергей Д.; Тодоровска, Елена Г.

Дата на публикация: 26.02.2010
3) Сертификат №: 10856 ЦАРЕВИЦА СОРТ ИНБРЕДНА ЛИНИЯ АНИС 102ИР

ZEA MAYS (L.) INBREDNA LINIIA ANIS 102 IR

Собственици: АГРОБИОИНСТИТУТ (АБИ) КОСТИНБРОД; АНИС ООД, ПЛЕВЕН

Автори: Христов, Николай К.; Христов, Кирил Н.; Атанасов, Атанас И.; Христова, Пенка И.; Влахов, Сергей Д.; Тодоровска, Елена Г.

Дата на публикация: 26.02.2010
4) Сертификат №: 10857 ЦАРЕВИЦА СОРТ ИНБРЕДНА ЛИНИЯ АНИС 101ИР

ZEA MAYS (L.) INBREDNA LINIIA ANIS 101 IR

Собственици: АГРОБИОИНСТИТУТ (АБИ) КОСТИНБРОД; АНИС ООД, ПЛЕВЕН

Автори: Христов, Николай К.; Христов, Кирил Н.; Атанасов, Атанас И.; Христова, Пенка И.; Влахов, Сергей Д.; Тодоровска, Елена Г.

Дата на публикация: 26.02.2010

      Journal Papers:

1.Kolev S, Ganeva G, Christov N, Belchev I, Kostov K, Tsenov N, Rachovska G, Landgeva S, Ivanov M, Abu-Mhadi N, Todorovska E (2010) Allele variation in loci for adaptive response and plant height and its effect on grain yield in wheat. Biotechnology & Biotechnological Equipment 24:2 1807-1813

2.Munsch M. A., Stamp P., Christov N. K., Foueillassar X. M., Husken A., Camp K.-H., Weider C., (2010) Grain yield increase and pollen containment by plus-hybrids could improve acceptance of transgenic maize, Crop Sci 50: 909-919

3.Ganeva G, Korzun V, Landjeva S, Popova Z, Christov N. K. (2010) Genetic diversity assessment of Bulgarian durum wheat (Triticum durum Desf.) landraces and modern cultivars using microsatellite markers, Genetic Resources and Crop Evolution, 57:2 273-285

4.Weider C., Stamp P., Christov N., Husken A., Foueillassar X., Camp K-H., Munsch M. (2009) Stability of Cytoplasmic Male Sterility in Maize under Different Environmental Conditions, Crop Science 49: 77-84

5.Todorovska E., Christov N., Slavov S., Christova P., Vassilev D. (2009). Biotic stress resistance in wheat – breeding and genomic selection implications. Biotechnol. & Biotechnol. Eq 23: 4, 1417-1426

6.Djilianov D., Ivanov S., Georgieva T., Moyankova D., Berkov S., Petrova G., Mladenov P., Christov N., Hristozova N., Peshev D., Tchorbadjieva M., Alexieva V., Tosheva A., Nikolova M., Ionkova I., van den Ende W. (2009). А holistic approach to resurrection plants.haberlea rhodopensis – А case study Biotechnol. & Biotechnol. Eq 23: 4, 1414-1416

7.Tsonev S., Todorovska E., Avramova V., Kolev S., Abu-Mhadi N., Christov N.K. (2009). Genomics assisted improvement of drought tolerance in maize: QTL approaches. Biotechnol. & Biotechnol. Eq 23: 4, 1410-1413

8.Dimitrova A., Todorovska E., Christov N., Stoilov L., Atanassov A., Gecheff K. (2008). Molecular characterization of DNA alterations induced by gamma-rays in barley genome. Genetics and Breeding 37:(3 - 4): 15-26

9.Christov N. K., Imai R., Blume Y.. (2008) Differential expression of two winter wheat alpha tubulin genes during cold acclimation, Cell Biology International 32:5, 574-578

10.Kostova A., Todorovska E., Christov N., Sevov V., Genov M., Vulchinkov S., Atanasov A. (2007) Assessing the genetic diversity of Bulgarian maize germplasm using microsatellite markers, MAYDICA 52:, 251-255

11.Zheleva D., Todorovska E., Christov N., Ivanov P., Ivanova I. and Todorov I. (2007) Assessing the genetic variation of Bulgarian bread wheat varieties by biochemical and molecular markers, Biotechnol. & Biotehnol. Eq 21:3 , 311-321

12.Christov, N.K. Yoneyama, S., Shimamoto, Y. and Imai, R. (2007) Differential expression of wheat genes during cold acclimation. Cytol. Genet. 41: 3, pp. 13-22

13.Christov I., Stefanov D., Velinov T., Goltsev V., Georgieva K., Abracheva P., Genova Y., Christov N. (2007) Study on symptomless leaf infection in grapevine plants grown in vitro with grapevine leafroll associated virus 3, Journal of Plant Physiology 164:9 1124-1133

14.Christova, P.K., Christov, N.K., Imai, R. (2006) A cold-inducible multidomain cystatin of winter wheat inhibits growth of the snow mold fungus, Microdochium nivale. Planta 223:6 1207 - 1218


  1. Zheleva D., Todorovska E., Jacquemin J.-M., Atanassov A., Christov N., Panayotov I., Tsenov N. (2006) Allele distribution at microsatellite locus Xgwm 261 marking the dwarfing gene Rht8 in hexaploid wheat from Bulgarian and Belgian gene bank collections and its application in breeding programs, Biotechnol. & Biotehnol. Eq 20:2 , 45-56

  2. Kostova А., Todorovska E., Christov N., Hristov K., Jacquemin J-M, Muhovski Y., Atanasov A. (2006) Genetic variation within and among populations of maize inbred B37 revealed by SSR markers, Biotechnol. & Biotehnol. Eq 20:2 , 37-44

  3. Kostova А., Todorovska E., Christov N., Sevov V., Atanasov A. (2006) Molecular characterisation of Bulgarian maize germplasm collection via SSR markers, Biotechnol. & Biotehnol. Eq 20:2 , 29-36

15.Kostova A., Todorovska E., Christov N., Hristov K., Atanasov A. (2006) Assesment of genetic variability induced by chemical mutagenesis in elite maize germplasm via SSR markers, Journal of Crop Improvement 16:1/2, 37 – 48

16.Костова А., Тодоровска Е., Христов Н., Вълчинкова П., Генов М., Атанасов А. (2006) Изследване на толерантни към засушаване български линии царевица с молекулни маркери, Растениевъдни Науки 39: 5, 50-55

17.Todorovska E., Abumhadi N., Kamenarova K., Jeleva D., Kostova A., Christov N.,
Alexandrova N., Jacquemin J-M., Anzai H., Nakamura C. and Atanassov A. (2005)
Biotechnological approaches for cereal crops improvement. Part II: Use of molecular markers in cereal breeding. Biotechnology and Biotechnological Equipment (Special issue)19: 3 91-104

18.Abumhadi N., Kamenarova K., Gecheff K., Christov N., Atanassov A (2005) Effect of chromosome reconstruction on tissue culture response of barley genome. Plant Tissue & Organ Culture 80: 339-342

19.Christov N., Todorovska E., Fasoula D., Ioannides I., Atanassov A. and Hristov K. (2004) Molecular characterization of chemical mutagenesis induced diversity in elite maize. Acta Biologica Yugoslavia, Genetica 36: pp. 47-60

20.Docheva P., Christov N., Batchvarova R., Atanassov A. (2003) Sexual crossing approach for combining multiple transgenes. Biotechnol. & Biotehnol. Eq., 17: 1 pp. 22-28.

21.Christov N., Christov I., Christov K. (2002) The model of ecologo-genetical organization of complex quantitative traits for productivity, resistance, and quality in plants., Biotechnology & Biotechnol. Eq., ., 16:2 (Supplement-Genetics & Challenges of 21st Century) , pp. 36-46

22.Abumhadi N., Trifonova A., Takumi S., Nakamura C., Todorovska E., Getov L.,


N. Christov, A. Atanassov (2001) Development of the particle inflow gun and optimising the particle bombardment method for efficient genetic transformation of immature embryos of cereals. , Biotechnology & Biotechnol. Eq., 15:2 (supplement) , pp. 87-96

23.Christov N., Marinova E., Ilchovska M., Ivanova M. and Christov K. (2000) Classical and biotechnological approaches to improve maize breeding in Bulgaria. Proceedings of XVIII Maize and Sorghum EUCARIA Conference, Genetika, 32:3, pp. 457-478 (2000)



  1. Гацовски Ж., Христов Н., Христов К. (1999) Екологогенетични аспекти на цитоплазмената мъжка стерилност при царевицата., Растениевъдни Науки 36, pp. 564-570

  2. Christov N., Imaishi H., Ohkawa H. (1999) Green-tissue-specific expression of a reconstructed cry1C gene encoding the active fragment of Bacillus thuringiensis δ-endotoxin in haploid tobacco plants conferring resistance to Spodoptera litura., Biosci. Biotechnol. Biochem., 63:8 pp. 1433-1444

  3. Христов K., Христов И., Христов Н. (1998) Прогресът на генетичния и молекулрно-генетичния анализ на количествени признак във връзка със селекцията на растенията, Селскостопанска Наука 3, pp. 13-21

  4. Christov N., Shiota N., Ohkawa H. (1998) Spodoptera litura tolerant transgenic tobacco plants expressing genetically modified cry 1 Ab genes from Bacillus thuringiensis., Biotechnology & Biotechnol. Eq., 1, pp. 8-16

  5. Иванова M., Петрова K., Вълчинкова П., Илчовска M., Христов K., Христов Н. (1996) Опити за ограничен пренос на гени при царевицата, чрез използване на облъчен с летални дози гама лъчи прашец E. mexicana Shrad. и Triplsacum dactiloides L., Научни Трудове на Селскостопанска Академия 3:1, pp. 43-45

  6. Christova P., Christov I., Christov N., Christov K. (1993) Chemical mutagenesis affects some quantitative traits and the zein composition of maize inbred lines; In A. Bianci, E. Lupotto and M. Motto (eds): Proceedings of the XVI Eucarpia Maize and Sorghum Conference, pp., Bergamo , Italy

  7. Christov N. K., Marinova E. I., Todorova L. G., Atanassov A. I. (1993) Protein and DNA markers in maize x tripsacum hybrids and their progenies.; In A. Bianci, E. Lupotto and M. Motto (eds): Proceedings of the XVI Eucarpia Maize and Sorghum Conference, pp., Bergamo , Italy



Conference Presentations



  1. Munsch M., Weider C., Christov N.K., Foueillassar X., Hüsken A., Camp K.-H., and Stamp P. (2009) Bio containment of maize by cytoplasmic male sterility and xenia. Co Extra International Conference 2-4 June, Paris (France) (oral)

  2. Hüsken A., Schiemann J., Fouiellassar X., Christov N., Munsch M., Camp K., Stamp P., Weider C. (2008) Cytoplasmic Male Sterility “bio-contains” transgenic pollen in maize 10th International Symposium on the Biosafety of Genetically Modifi ed Organisms, 16-21 Nov., Wellington, New Zealand (poster)

  3. Weider, C., Camp K-H., Christov N., Hüsken A., Fouiellassar X. and Stamp P. (2007) GM pollen containment by cytoplasmic male sterility (CMS) in maize (Zea mays L.) GMCC-07: Third International Conference on Coexistence between Genetically Modified (GM) and non-GM based Agricultural Supply Chains, 20-21 Nov. Seville (Spain) (poster)

  4. Munsch, M., Camp K.-H., Christov N., Hüsken A., Fouiellassar X. and Stamp P. (2007) The Plus-Hybrid system in maize: biocontainment of transgenic pollen and grain yield increase GMCC-07: Third International Conference on Coexistence between Genetically Modified (GM) and non-GM based Agricultural Supply Chains, 20-21 Nov. Seville (Spain) (poster)

  5. Todorovska E., Zheleva D., Christov N., Jacquemin J., Fasoula D., Ioannides I., Bozhanova V., Dechev D., A. Atanassov (2007). The molecular diversity of Bulgarian cereal germplasm collections – a reference point for better understanding and broadening of the genetic base of cereal crops. Proceedings of the 1st workshop on TritiGenCOST action FA0604: Triticeae genomics for the advanced of essential European crops, Book of Abstracts, P1.1, pp. 39, 1-2 October, 2007, Puerto de la Crus, Tenerife, Canary Islands, Spain. (poster)

  6. Christov N., Christova P. , Kato H., Imai R. (2007). Identification of cDNAs involved in winter wheat cold acclimation process. Proceedings of the 1st workshop on TritiGenCOST action FA0604: Triticeae genomics for the advanced of essential European crops, Book of Abstracts, pp.50, 1-2 October, 2007, Puerto de la Crus, Tenerife, Canary Islands, Spain (oral).

  7. Christova P., Christov N., Imai R. (2007). Functional analysis of antifungal activities in a wheat multidomain cystatinTaMDC1 Proceedings of the 1st workshop on TritiGenCOST action FA0604: Triticeae genomics for the advanced of essential European crops, Book of Abstracts, pp.50, 1-2 October, 2007, Puerto de la Crus, Tenerife, Canary Islands, Spain (oral).

  8. Todorovska E., Zheleva D., Christov N., Jacquemin J., Fasoula D., Ioannides I., Bozhanova V., Dechev D., A. Atanassov (2007). The molecular diversity of Bulgarian cereal germplasm collections – a reference point for better understanding and broadening of the genetic base of cereal crops. 6th Plant Genomics European Meetings, Book of Abstracts, pp. 148, P04.7, 3-6 October, 2007, Tenerife, Spain

  9. Todorovska E., Ganeva G, Korzun V., Zheleva D., Prokopova K., Landjeva S., Christov N., N. Tzenov (2007). Distribution of semi-dwarf (Rht) alleles among Bulgarian wheat cultivars and their role on grain yield. 6th Plant Genomics European Meetings, Book of Abstracts, P 04.7, pp. 147, 3-6 October, 2007, Tenerife, Spain

  10. Zheleva D.D., Kostova A. R., Todorovska E. G, Christov N. K. and A. I. Atanassov (2006). Characterization of Bulgarian cereal germplasm by SSR markers. Plant Genomics European Meetings. Book of abstracts pp.341, 11-14 October, Venice, Italy.

  11. Christov N., Christova P., Imai R.
    Functional analyses of cold acclimation induced genes in wheat International Symposium: “Biosafety Issues in Implementation of GMOs: New Research Approaches, Regulation, and Public Perception”, Yalta, Ukraine 10–14 May 2006 (oral)

  12. Christova P., Christov N., Imai R.
    A cold inducible multidomain cystatin from winter wheat inhibits growth of snow mold fungus, Microdochium nivale. 47th Annual Meeting of the Japanese Society of Plant Physiologists, Tsukuba, Japan, 19-21.March, 2006, Plant Cell Physiol 47:083 (Supplement) (1aJ03)(oral)

  13. Christov N., Christova P., Kato H. Imai R.
    Overexpression of a shaggy-like kinase from wheat enhances drought tolerance of Arabidopsis by activating a novel signalling pathway. 47th Annual Meeting of the Japanese Society of Plant Physiologists, Tsukuba, Japan, 19-21.March, 2006, Plant Cell Physiol 47:789 (Supplement) (P243) (poster)

  14. Kostova A, Todorovska E, Christov N, Sevov V, Hristov K, Genov M, Valchinkov S, Atanassov A
    Assessing the genetic diversity of Bulgarian maize germplasm using microsatellite markers. Intl. Maize Conference, “60th anniversary Maize Research Institute Zemun Polje”, Oct. 26-28 2005, Belgrade, Serbia and Montenegro(oral)

  15. Christov N., Christova P. and R. Imai,
    Macroarray approach for identification of novel genes involved in cold acclimation in Triticum aestivum, (poster session), 7-th IPCHS, July 10-15, 2004, Sapporo, Japan

  16. E. Todorovska, N. Christov, V. Milkova, D. Fasoula, I. Ioannides, J.M. Jacquemin, B. Bojinov, A. Atanassov
    Genetic diversity assessment among Bulgarian and western European wheat germplasmcollections using microsatellite markers J. Vollmann, H. Grausgruber & P. Ruckenbauer (eds.), 2004. Genetic variation for plant breeding, Proceedings of the 17th EUCARPIA General Congress, 8 - 11 September 2004, Tulln, Austria. BOKU – University of Natural Resources and Applied Life Sciences, Vienna, Austria.p.142 (poster)

  17. E. Todorovska, N. Christov, V. Milkova, D. Fasoula, I. Ioannides, J.M. Jacquemin, A. Graner, A. Atanassov
    Characterization of Bulgarian and Cypriot barley germplasm collections by microsatellite markers J. Vollmann, H. Grausgruber & P. Ruckenbauer (eds.), 2004. Genetic variation for plant breeding, Proceedings of the 17th EUCARPIA General Congress, 8 - 11 September 2004, Tulln, Austria. BOKU – University of Natural Resources and Applied Life Sciences, Vienna, Austria. p.149 (poster)

  18. Todorovska, E. Christov, N., Milkova, V. Atanassov, A., Bojinov, B., Jacquemin, J., M., Fasoula,D., Ioannidou, I.
    Genotyping of wheat and barley Germplasm collections by SSR markers. Bulgarian- Russian seminar “The application of molecular diagnostic in breeding variety testing and analysis of transgenic plants” pp26 , 3-7 July 2004, Varna, Bulgaria

  19. N. Christov, E. Todorovska, D. Fasoula, I. Ioannides, A. Atanassov, K. Hristov
    Molecular characterization of chemical mutagenesis induced (diversity) variability in elite maize germplasm, XIX Maize and Sorghum EUCARIA Conference“Maize and sorghum genomics and breeding”, June, 4-7, 2003, Barcelona, Spain (oral)

  20. K. Христов , Н. Христов, И. Христов
    Успехи на генетиката и значението им за селекцията на растенията, Юбилейна научна сесия “110 години въздухоплаване в България” 25-26 април 2002, ВВВУ “Г. Бенковски”, Долна Митрополия

  21. N. Christov
    Particle bombardment mediated transformation- a tool for cereal breeding and functional genomics. International conference: “Biotechnology approaches for exploration and preservation of plant genetic resources”, May. 26-31, 2002, Yalta, Ukraine

  22. N. Abumhadi, A. Trifonova, S. Takumi, C. Nakamura, E. Todorovska, L. Getov, N. Christov, A. Atanassov
    Development of the particle inflow gun and optimising the particle bombardment method for efficient genetic transformation of immature embryos of cereals, Turkish-Bulgarian workshop on plant biotechnology, May 27-30, 2001, TUBITAK, Maramara research center, Gebze- Kocaeli, Turkey

  23. N. Christov, N. Abumhadi, A. Trifonova, I. Malchev, T. Iankova, A. Atanassov
    Genetic improvement of cereal crops through biotechnology. International conference: “Plant biotechnology facing the new millennium”, Oct. 16-18, 2000, Kostinbrod, Bulgaria

  24. K. Hristov, N. Christov, I. Christov, M. Ilchovska, M. Ivanova
    Biotechnological and ecologo-genetic approaches in the technology for mutation breeding of maize. International conference “Plant biotechnology facing the new millennium”, Oct. 16-18, 2000, Kostinbrod, Bulgaria

  25. N. Abumhadi, A. Trifonova, I. Malchev, T. Iankova, A. Atanassov, N. Christov
    An efficient and albino-free regeneration system from mature embryos of elite Bulgarian brewing barley cultivars (Hordem vulgare L.). International conference: “Plant biotechnology facing the new millennium”, Oct. 16-18, 2000, Kostinbrod, Bulgaria

  26. N. Abumhadi, A. Trifonova, I. Malchev, T. Iankova, A. Atanassov, N. Christov
    Production of fertile transgenic Bulgarian brewing barley (Hordem vulgare L.) cultivars by particle bombardment of mature embryos. International conference: “Plant biotechnology facing the new millennium”, Oct. 16-18, 2000, Kostinbrod, Bulgaria

  27. N. Christov, E. Marinova, M. Ilchovska, M. Ivanova, K. Christov
    Classical and biotechnological approaches to improve maize breeding in Bulgaria. XVIII Maize and Sorghum EUCARIA Conference, June, 4-9, 2000, Sava Center, Beogradе, Yugoslavia, (invited speaker)

  28. Ж. Гацовски, Н. Христов, К. Христов
    Еколого-генетични аспекти на цитоплазмената мъжка стерилност при царевицата., Юбилейна сесия 75 г. ИЦ-Кнежа 09.09,1999, Кнежа

  29. Christov N., Imaishi H., Ohkawa H.
    Green tissue specific expression of a truncated cry1C gene from Bacillus thuringiensis in haploid tobacco plants 407 Annual Meeting of West Japan Biosci. Biotech. Biochem. Society Dec. 5 1998, Kobe, Japan

  30. Christov N., Imaishi H., Ohkawa H.
    Transgenic tobacco plants expressing simultaneously Bacillus thuringiensis cry1C and rat P450 1A1/yeast P450 reductase fusion gene are both insect and herbicide tolerant., 1998 Symposium of Biosci. Biotech. Biochem. Society of Japan, Mar., 31-April, 3 1998, Nagoya, Japan

  31. Christov N., Imaishi H., Ohkawa H.
    Cytochrome P450 as a selection marker for tobacco transformation., BRAIN Seminar, Oct. 30-31, 1997, Tsukuba, Ibaraki, Japan

  32. Christov N., Ohkawa H.
    Engineering of insect resistant tobacco plants expressing modified cryIA(b) genes from Bacillus thuringiensis ssp. aizawai, 392 Annual Meeting of West Japan Biosci. Biotech. Biochem. Society Dec. 7 1995, Kobe, Japan

  33. Christova P., Christov I, Christov N., Christov K.
    Chemical mutagenesis affects some quantitative traits and the zein composition of maize inbred lines, The XVIth Conference of the Eucarpia Maize and Sorghum Section, June 6-9 1993, Bergamo, Italy

  34. Christov N. K., Marinova E. I., Todorova L. G., Atanassov A. I.
    Protein and DNA markers in maize x tripsacum hybrids and their progenies, The XVIth Conference of the Eucarpia Maize and Sorghum Section, June 6-9 1993, Bergamo, Italy

  35. Christov N. K., Marinova E. I., Todorova L. G., Atanassov A. I
    Protein markers candidates for evaluation of the presence of germplasm from Tripsacum in Zea x Tripsacum hybrid derivatives.; 6th FAOB Congress. 16-21 Nov. , 1992. Shanghai, China.

  36. Christov N., Marinova E., Doncova P., Todorova L., Atanassov A.
    PAGE Analysis of Prolamine Proteins in Zea Mays L., Tripsacum dactyloides L., Maize x Tripsacum Hybrids and Lines.; Symp. Biochemical Genetics of Plants. 9-10 Oct. 1991 Sofia, Bulgaria.



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