Департамент обща микробиология ръководител

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  Генетична комплементация на на мутацията ade3-5 и идентифициране на ген PUR2,5 от пуриновата биосинтеза на метилотрофни дрожди Hansenula (Ogataea) polymorpha. Генът кодира бифункционален белтък с глицинамид-риботид синтетазна и аминоимидазол-риботид синтетазна активности, катализиращи съответно втория и петия етап от de novo пуриновата биосинтеза в този вид дрожди.
  
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  Конструиране на совалкови вектори за E. coli и Klebsiella, хетероложна експресия на амилазен ген на Bacillus licheniformis под контрола на lac-промотор и успешна секреция на ензима в двата гостоприемника.
  
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  Клониране, секвениране и хетероложна експресия на cgt гени, кодиращи циклодекстрин-глюканотрансферази от алкалофилни бацили в E. coli.
  
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  Идентифициране, клониране и секвениране на гени за амилаза, амилопулуланаза и инулиназа от различни родове млечнокисели бактерии.
  

Tsvetanova F., Petrova P., Petrov K. (2014) 2,3-butanediol production from starch by engineered Klebsiella pneumoniae G31-A. Applied Microbiology and Biotechnology, vol. 98, 2441-2451.

Stoyanov A., Petrova P., Lahtchev K. (2014) Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha, Journal of BioScience and Biotechnology, vol. 3(3), 247-252.

Velikova P., Blagoeva G., Gothcheva V., Petrova P. (2014) Novel Bulgarian Lactobacillus strains ferment prebiotic carbohydrates, Journal of BioScience and Biotechnology, SE/Online, 55-60.

Ivanova V., Petrov K., Safarikova M., Safarik I., Petrova P., Tonkova A. Delchev N. (2014) Immobilization of recombinant CGTase JCGT8-5 on magnetically – modified silicates and natural supports. International Review of Chemical Engineering, vol. 6 (1), 1-8.

Petrova P., Petrov K., Stoyancheva G. (2013) Starch-modifying enzymes of lactic acid bacteria – structures, properties, and applications. Starch-Starke vol. 65 (1/2), 34-47.

Petrova P., Tomova I., Petrov K., Nikov I., Tonkova A. (2013) Purification and properties of a new recombinant cyclodextrin glucanotransferase from E. coli BL21 (DE3) pJCGT8-5. Comptes Rendus de l'Academie Bulgare des Sciences vol. 66 (10), 1437-1444.

Blagoeva G., Petrova P., Petrov K., Gotcheva V., Angelov A. (2013) Amylolytic probiotics with application in food industry. ENGIHR The Intestinal Microbiota and Gut Health: Contribution of the Diet, Bacterial Metabolites, Host Interactions and Impact on Health and Disease Valencia, Spain 18^th-20^th September 2013, p. 28-32.

Petrova P., Petrov K. (2012) Direct starch conversion into L (+) lactic acid by a novel amylolytic strain of Lactobacillus paracasei B41. Starch-Starke vol. 64 (1), 10-17.

Petrova P., Tonkova A., Petrov K. (2012) Sequence analysis, cloning and extracellular expression of cyclodextrin glucanotrasferase gene from the alkaliphilic Bacillus pseudalcaliphilus 8SB in E. coli, Process Biochemistry vol. 47 (12), 2139-2145.

Ivanova I., Tonkova A., Petrov K., Petrova P., Gencheva P. (2012) Covalent attachment of cyclodextrin glucanotransferase from genetically modified Escherichia coli on surface functionalized silica coated carriers and magnetic particles, Journal of BioScience and Biotechnology, vol. 1 (SE), 7-13.

Petrova P., Tsvetanova F., Petrov K. (2012) Regulation of amylase genes expression in lactic acid bacteria. New Trends in Microbiology, In: 65th Anniversary of the Stephan Angeloff Institute of Microbiology, pp. 193-203. (ISBN 978-954-92882-1-6)

Lachtchev K., Petrova P., Lyutskanova D., Stoyanov A. (2012) Biotechnological potential of methylotrophic yeasts. New Trends in Microbiology, In: 65th Anniversary of the Stephan Angeloff Institute of Microbiology, pp. 265-279. (ISBN 978-954-92882-1-6)

Kitayska T., Petrova P., Tonkova A., Ivanova V. (2011) Purification and properties of a new thermostable cyclodextrin glucanotransferase from Bacillus pseudalcaliphilus 8SB, Applied biochemistry and biotechnology, vol. 165, 1285–129.

Petrova P., Petrov K. (2011) Antimicrobial activity of starch-degrading Lactobacillus strains isolated from boza. Biotechnology & Biotechnological Equipment vol. 25 (4), 114-116.

Petrov K., Petrova P. (2010) Enhanced production of 2,3-butanediol from glycerol by forced pH fluctuations, Applied Microbiology and Biotechnology, vol. 87 (3), 943–949.

Petrova P., Emanuilova M., Petrov K. (2010) Amylolytic Lactobacillus Strains from Bulgarian fermented beverage Boza, Zeitschrift für Naturforschung, vol. 65c (3-4), 218-224.

Petrova P., Petrov K. (2010) Amylolytic lactic acid bacteria and their industrial application. Journal of International Scientific Publications: Materials, Methods & Technology, vol. 4 (1), 349-358.

СЪСТАВ

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  Биоразнообразие на дрожди в избрани Български екосистеми., TK-176, финансиран от Фонд Научни Изследвания 2008-2012 г.
  

Dimitrov, R., Gouliamova, D. Biological sequence comparison, molecular evolution and phylogenetics. (2012). Biotech. Biotech. Equip. v. 26 (1):207-219.

Gouliamova, D., Stoilova-Disheva, M., Dimitrov, R., Gushterova, A., Vasileva-Tonkova, E., Paskaleva, D., Stoyanova, P. Preliminary characterization of yeasts and actinomycetes isolated from mammalian feces. (2012). Biotech. Biotech. Equip v. 26 (1):1-4.
Gouliamova, D., Dimitrov, R., Stoilova-Disheva, M. DNA barcoding of yeasts from selected Bulgarian food products. (2012). Biotech. Biotech. Equip. v. 26(1): 32-34.
Hristov, A., Gouliamova, D., Nacheva, L., Tsekova, K. Biodegradation of phenol in the precence of heavy metals by free and immobilised cells of yeast associations. (2012). Comptes rendus de l’Acade'mie bulgare des Sciences. v. 3:335-340.
Gouliamova, D. E. and Hennebert, G.L. Phylogenetic relationships in Saccharomyces cerevisiae complex species. (2010). In the book 100 Years of the Fungal Collection MUCL 1894-1994. Fungal taxonomy and tropical mycology: Quo vadis? (Editor G.L. Hennebert). Catholic University of Louvain, Belgium. Published by Mycotaxon, Ltd. Ithaca, New York, USA. ISBN 078-0-930845-18-6. pp.172-175.
Péter, G., Dlauchy, D., Tornai-Lehoczki, J., Gouliamova, D., Kurtzman, C.P. Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. (2011). Antonie Van Leeuwenhoek. 100(3):375-83.
Dimitrov, R. A., Gouliamova, D. E. Bioinformatics education: perspective and challenges. (2009). Biotech. & Biotec. Equip. 23: 40-42.
Gouliamova, D. E., Dimitrov, R. A., Petrova, P. M., Stoyancheva, G. D. Genomic approaches to yeast taxonomy. (2009). Biotech. & Biotech. EQ. 23: 519-521
Доклади

Gouliamova, D., Dimitrov R., Groenewald, M., Smith, M. Th., Boekhout T. DNA barcoding analysis of yeasts from selected Bulgarian ecosystems. Oral presentation. International congress ICY-2012, “Yeasts for sustainable future” Madison, Wisconsin, The USA, 24-31 August, 2012.

Boekhout, T. Biodiversity of yeasts endosymbionts in Bulgarian Coleoptera. The 9th

International Mycological Congress, IMC9 “The Biology of Fungi”, Edinburgh, UK, 1-6 August, 2010.

Секция МИКРОБНА БИОХИМИЯ
Ръководител: Доц. Евгения Василева-Тонкова, д-р +359 2 979 31 39, evaston@yahoo.com
СЪСТАВ

Доц. Блага Ангелова, д-р +359 2 979 31 54, blaga@microbio.bas.bg 

 Гл. Асистент Нели Христова, д-р, +359 2 979 31 15, nchrist@abv.bg

 Асистент Анна Сотирова +359 2 979 31 39, anna@microbio.bas.bg 

 Асистент Стефан Енгибаров +359 2 979 31 47, stefan_engibarov@abv.bg 

 Асистент Румяна Енева +359 2 979 31 47, rum_eneva@abv.bg 

 Асистент Ирина Лазаркевич +359 2 979 3139, irinalazarkevich@abv.b
Изследователска дейност на секцията

Ензими, продуцирани от микроорганизми: изолиране, пречистване, свойства и биологична роля. Микробна трансформация на стероидни съединения. Биологично-активни съединения от микробен и синтетичен произход – антимикробна активност, механизъм на действие. Сърфактанти, продуцирани от микроорганизми (биосърфактанти) – биосинтез, изолиране, пречистване, физико-химични свойства; влияние върху повърхностните свойства на микробната клетка; приложение в биотехнологии, в медицината, фармакологията и опазване на околната среда. Характеристика и изучаване на метаболитните способности на нови мезофилни и психрофилни/психротолерантни бактерии и дрожди, изолирани от екстремни екологични ниши за получаване на ензими, антимикробни вещества и др. Микробна деградация на алифатни и ароматни въглеводороди – замърсители на околната среда.

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  Mикробно разнообразие в българската пещера Магурата и опазване на уникалните скални рисунки. Договор №: ДДВУ 02/73-2010, ФНИ.
  

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  Биоразнообразие на антарктични микробни съобщества и тяхната адаптация към екстремните обкръжаващи условия. Договор №: ДНТС Украйна 01/1-2012, ФНИ.
  

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  Получаване на биологично-активни съединения от микробен и синтетичен произход за нови фармацефтични препарати, за биологично разграждане на отпадъци и други биотехнологии” Договор №: ДНТС Украйна 01/0003/ 2012, ФНИ.
  

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  Изолиран е нов вид бактерия, Myroides guanonis, от праисторическите рисунки в пещера Магурата.
  

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  Avramova, T., Sotirova, A., Galabova, D., Karpenko, E., 2008, Effect of Triton X-100 and rhamnolipid PS-17 on the mineralization of phenanthrene by Pseudomonas sp. cells. Intern.Biodeter.Biodegr., 62, 415-420.
  
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  Mutafov, S., Avramova, T., Angelova, B., Stefanova, L., 2008, Temperature effect on the azo bond reduction kinetics of a sulfonated mono azo dye. Biodegradation., 19, 387-389.
  
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  Sotirova, A., Spasova, D., Galabova, D., Karpenko, E., Shulga, A., 2008, Rhamnolipid–biosurfactant permeabilizing effects on Gram-positive and Gram-negative bacterial strains. Curr. Microbiol., 56, 639-644,.
  
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  Sotirova, A., D. Spasova, E. Vasileva-Tonkova, D. Galabova, 2009, Effects of rhamnolipid- biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiol. Res., 164 (3), 297-303.
  
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  Tuleva, B., Christova, N. , Cohen, R., Antonova, D., Todorov, T., Stoineva I., 2009, Isolaton and charaterization of trehalose tetraester biosurfactants from a soil strain Micrococcus luteus BN56., Process Biochem., 44, 135-141.
  
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  Vasileva-Tonkova, E., Sotirova, A., Galabova, D., 2011, The effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on model bacterial strains and isolates from industrial wastewater. Curr. Microbiol., 62, 427-433.
  
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  Gousterova, А., Nustorova, М., Paskaleva, D., Naydenov, M., Neshev, G., Vasileva-Tonkova, E., 2011, Assessment of feather hydrolysate from thermophilic actinomycetes soil amendment and biological control application. Int. J. Environ. Res. 5, 1065-1070.
  
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  Christova, N., Tuleva, B., Cohen, R., Ivanova, G., Stoev, G., Stoilova-Disheva, M., Stoineva I., 2011, Chemical characterization, physical and biological activities of rhamnolipids produced by Pseudomonas aeruginosa BN10. Z. Naturforsch., 66C, 394-395.
  
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  Eneva, R., Engibarov, S., Strateva, T., Abrashev, R., Abrashev, I., 2011,Biochemical studies on the production of neuraminidase by environmental isolates of Vibrio cholerae non-O1 from Bulgaria. Can. J. of Microbiol., 57, 606-610.
  
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  Gesheva, V., Vasileva-Tonkova, E., 2012, Production of enzymes and antimicrobial compounds by halophilic Antarctic Nocardioides sp. grown on different carbon sources. World J. Microbiol. Biotechnol., 28, 2069-2076.
  
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  Sotirova, A., Avramova, T., Stoitsova, S., Lazarkevich, I., Lubenets, V., Karpenko, E., Galabova, D., 2012, The importance of rhamnolipid- biosurfactant induced changes in bacterial membrane lipids of Bacillus subtilis for the antimicrobial activity of thiosulfonates, Curr. Microbiol., 65, 534-541.
  
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  Christova, N., Petrov, P., Kabaivanova, L., 2013, Biosurfactant production by entrapped in cryogels Pseudomonas aeruginosa BN10 cells. Z. Naturforsch., 68c, 47-52.
  
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  Christova, N., Stoineva, I., Trehalose biosurfactants, Biosurfactants: Research Trends and Applications , Chapter 9, Editors , Milligan C.N., Sharma S., Mudhoo A. CRC Press (Taylor & Francis)
  
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  Christova, N., Tuleva, B., Kril, A., Georgieva, M., Konstantinov, S., Terziyski, I., Nikolova, B., Stoineva, I., 2013, Chemical structure and in vitro antitumor activity of rhamnolipids from Pseudomonas aeruginosa BN10. Appl. Biochem. Biotechnol., 170, 676–689.
  
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  Ivanova, V., Tomova, I., Kamburov, A.,Tomova, A., Vasileva-Tonkova, E., Kambourova, M., 2013, High phylogenetic diversity of bacteria colonizing prehistorical paintings in Magura Cave, Bulgaria. J Caves Karst Stud., 75, 218-228.
  
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  Tomova, A., Tomova, I., Vasileva-Tonkova, E., Lazarkevich, I., Stoilova-Disheva, M., Lyutskanova, D., Kambourova, M., 2013, Myroides guanonis sp. nov., isolated from prehistoric paintings. Int. J. Syst. Evol. Microbiol., 63, 4266-4270.
  
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  Tomova, I., Lazarkevich, I., Tomova, A., Kambourova, M., Vasileva-Tonkova, E., 2013, Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria. Int. J. Speleol., 42, 65-76.
  
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  Engibarov, S., Eneva, R., Abrashev, I., 2014, Neuraminidase (sialidase) from an Aeromonas sp. strain A40/02 – isolation and partial purification. Ann. Microbiolol,, 10, doi: 10.1007/s13213-014-0990-0
  
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  Galabova, D., Sotirova, A., Karpenko, E., Karpenko, O., 2014, Role of Microbial Surface-Active Compounds in Environmental Protection. The Role of Colloidal Systems in Environmental Protection, 41-83,
  
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  Gousterova, A., Paskaleva, D., Vasileva-Tonkova, E., 2014, Characterization of culturable thermophilic actinobacteria from Livingston Island, Antarctica. Int. Res. J. Biol. Sci., 3, 30-36.
  
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  Hristov, A., Christova, N., Nacheva, L., Stoineva, I., Kabaivanova, L., 2014, Biodegradation potential of Nocardia farcinica BN26 for xenobiotics mineralization. Cr. Acad. Bulg. Sci., 67, 821-830.
  
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  Sotirova, A., Avramova, T., Lazarkevich, I., Lubenetz, V., Karpenko, O., Galabova, D., 2014, Antibacterial Potential of Novel Synthetic Derivatives of 1, 4-Naphthoquinone and Their Complexes with Biosurfactants. Res. J. Pharm. Biol. Chem. Sci., 5, 530-541
  
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  Terziyski, I., Stoineva, I.,Christova, N., Alexandrova, L., Todorov, R., Cohen, R., 2014,Foam and wetting films from rhamnolipids produced by Pseudomonas aeruginosa BN10. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 299-305.
  
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  Tomova I., Gladka G., Tashyrev, A., Vasileva-Tonkova, E., 2014, Isolation, identification and hydrolytic enzymes production of aerobic heterotrophic bacteria from two Antarctic islands. Int. J. Environ. Sci., 4, 614-625.
  
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  Tomova, I., Stoilova-Disheva, M., Vasileva-Tonkova, E., 2014, Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica. Polish Polar Res., 35, 593-607.
  
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  Vasileva-Tonkova, E., Romanovskaya, V., Gladka, G., Gouliamova, D., Tomova, I., Stoilova-Disheva, M., Tashyrev, O., 2014, Ecophysiological properties of cultivable heterotrophic bacteria and yeasts dominating in phytocenoses of Galindez Island, maritime Antarctica. World J. Microbiol. Biotechnol., 30, 1387-1398.